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Literature summary extracted from

  • Lee, S.G.; Kim, Y.; Alpert, T.D.; Nagata, A.; Jez, J.M.
    Structure and reaction mechanism of phosphoethanolamine methyltransferase from the malaria parasite Plasmodium falciparum: an antiparasitic drug target (2012), J. Biol. Chem., 287, 1426-1434.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.103 medicine the enzyme is an antiparasitic target Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.103 in complex with either S-adenosyl-L-methionine (1), phosphoethanolamine (2), phosphocholine (3), sinefungin (4), or both phosphoethanolamine and S-adenosylhomocysteine (5), hanging drop vapor diffusion method, using (1) 20% (w/v) PEG-8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl) phosphine, or (2-5) 20-30% (w/v) PEG8000, 0.1 M sodium cacodylate,pH 6.5, 0.2 M sodium acetate, 5 mM 2-mercaptoethanol Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.103 D110A the mutant shows about 37% of wild type activity Plasmodium falciparum
2.1.1.103 D110N the mutant shows about 80% of wild type activity Plasmodium falciparum
2.1.1.103 D61A the mutant shows about 35% of wild type activity Plasmodium falciparum
2.1.1.103 D61N the mutant shows about 68% of wild type activity Plasmodium falciparum
2.1.1.103 D85A inactive Plasmodium falciparum
2.1.1.103 D85N inactive Plasmodium falciparum
2.1.1.103 H132A inactive Plasmodium falciparum
2.1.1.103 H132N inactive Plasmodium falciparum
2.1.1.103 H132Q inactive Plasmodium falciparum
2.1.1.103 I86A the mutant shows about 10% of wild type activity Plasmodium falciparum
2.1.1.103 I86F the mutant shows about 16% of wild type activity Plasmodium falciparum
2.1.1.103 K247A inactive Plasmodium falciparum
2.1.1.103 K247M inactive Plasmodium falciparum
2.1.1.103 Q18N the mutant shows about 20% of wild type activity Plasmodium falciparum
2.1.1.103 R179A inactive Plasmodium falciparum
2.1.1.103 R179A the mutant shows about 5% of wild type activity Plasmodium falciparum
2.1.1.103 R179K the mutant shows about 45% of wild type activity Plasmodium falciparum
2.1.1.103 S37A the mutant shows about 85% of wild type activity Plasmodium falciparum
2.1.1.103 Y160A inactive Plasmodium falciparum
2.1.1.103 Y160F inactive Plasmodium falciparum
2.1.1.103 Y175A the mutant shows about 10% of wild type activity Plasmodium falciparum
2.1.1.103 Y175F the mutant shows about 9% of wild type activity Plasmodium falciparum
2.1.1.103 Y181A the mutant shows about 7% of wild type activity Plasmodium falciparum
2.1.1.103 Y181F the mutant shows about 5% of wild type activity Plasmodium falciparum
2.1.1.103 Y19A inactive Plasmodium falciparum
2.1.1.103 Y19F the mutant shows about 30% of wild type activity Plasmodium falciparum
2.1.1.103 Y27A the mutant shows about 5% of wild type activity Plasmodium falciparum
2.1.1.103 Y27F the mutant shows about 8% of wild type activity Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.103 sinefungin
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.0299
-
 S-adenosyl-L-methionine using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 0.0331
-
 S-adenosyl-L-methionine using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 0.0542
-
 Phosphoethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 0.0668
-
 phosphodimethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 0.181
-
 phosphomonomethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Plasmodium falciparum Q6T755
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 nickel-affinity column chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 3 S-adenosyl-L-methionine + phosphoethanolamine overall reaction Plasmodium falciparum 3 S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + phosphomonomethylethanolamine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphomonomethylethanolamine
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + phosphodimethylethanolamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.103 phosphoethanolamine methyltransferase
-
 Plasmodium falciparum
2.1.1.103 PMT
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.103 0.39
-
 S-adenosyl-L-methionine using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 0.49
-
 S-adenosyl-L-methionine using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 1.82
-
 Phosphoethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 3.08
-
 phosphodimethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 3.63
-
 phosphomonomethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.103 13.1
-
 S-adenosyl-L-methionine using phosphoethanolamine as cosubstrate pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 14.7
-
 S-adenosyl-L-methionine using phosphodimethylethanolamine as cosubstrate, pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 20.07
-
 phosphomonomethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 33.52
-
 Phosphoethanolamine pH and temperature not specified in the publication Plasmodium falciparum
2.1.1.103 46.16
-
 phosphodimethylethanolamine pH and temperature not specified in the publication Plasmodium falciparum