EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.2.2 | expressed in Pichia pastoris and in HEK-293 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.2 | N120Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N230Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N266Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N297Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a slightly decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N344Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N511Q | the mutant exhibits a Km value for the transpeptidation reaction that is not significantly different from that of wild type enzyme. The mutation results in a decreased maximal rate of 5-L-glutamyl-4-nitroanilide turnover | Homo sapiens |
2.3.2.2 | N95Q | the mutation results in an 8fold decrease in the cleavage efficiency of the propeptide | Homo sapiens |
2.3.2.2 | N95Q/N120Q/N230Q/N266Q/N297Q/N344Q/N511Q | total N-glycosylation knock-out mutant | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.2 | 1.1 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N266Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.2 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N95Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N120Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N230Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N297Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N344Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme N511Q, at 37°C, pH 7.4 | Homo sapiens | |
2.3.2.2 | 1.3 | - |
5-L-glutamyl-4-nitroanilide | wild type enzyme, at 37°C, pH 7.4 | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.2.2 | membrane | - |
Homo sapiens | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 22000 | - |
1 * 64000 + 1 * 22000, SDS-PAGE | Homo sapiens |
2.3.2.2 | 64000 | - |
1 * 64000 + 1 * 22000, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.2 | additional information | Homo sapiens | in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.2 | Homo sapiens | P19440 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.3.2.2 | glycoprotein | autocatalytic cleavage of human gamma-glutamyl transpeptidase is highly dependent on N-glycosylation at asparagine 95. The large subunit of the enzyme contains six N-glycosylation sites (Asn-95, -120, -230, -266, -297, and -344), and the small subunit contains a single site (Asn-511) | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.2 | 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 5-L-glutamyl-glycylglycine + 4-nitroaniline | - |
? | |
2.3.2.2 | additional information | in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.2 | heterodimer | 1 * 64000 + 1 * 22000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.2 | gamma-glutamyl transpeptidase | - |
Homo sapiens |
2.3.2.2 | GGT | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 37 | 77 | in both the transpeptidation and hydrolysis reactions, wild type enzyme exhibits full activity up to 47°C. At temperatures greater than 62°C, the wild type enzyme is rendered completely inactive | Homo sapiens |