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Literature summary extracted from

  • Malle, E.; Zhou, H.; Neuhold, J.; Spitzenberger, B.; Klepsch, F.; Pollak, T.; Bergner, O.; Ecker, G.; Stolt-Bergner, P.
    Random mutagenesis of the prokaryotic peptide transporter YdgR identifies potential periplasmic gating residues (2011), J. Biol. Chem., 286, 23121-23131.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.4.2.5 homology modeling based on the crystal structure of the Shewanella oneidensis peptide transporter PepTso, identifies Glu56 and Arg305 as potential periplasmic gating residues Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.5 A123V partial loss of uptake Escherichia coli
7.4.2.5 A264P partial loss of uptake Escherichia coli
7.4.2.5 A285V complete loss of uptake Escherichia coli
7.4.2.5 A303G partial loss of uptake Escherichia coli
7.4.2.5 A68P complete loss of uptake Escherichia coli
7.4.2.5 E56G complete loss of uptake Escherichia coli
7.4.2.5 F197I change in selectivity Escherichia coli
7.4.2.5 F289L complete loss of uptake Escherichia coli
7.4.2.5 F289S complete loss of uptake Escherichia coli
7.4.2.5 F301I complete loss of uptake Escherichia coli
7.4.2.5 G101D partial loss of uptake Escherichia coli
7.4.2.5 G127D partial loss of uptake Escherichia coli
7.4.2.5 G78C complete loss of uptake Escherichia coli
7.4.2.5 G86R partial loss of uptake Escherichia coli
7.4.2.5 I100V no membrane localization Escherichia coli
7.4.2.5 I122N partial loss of uptake Escherichia coli
7.4.2.5 I60N complete loss of uptake Escherichia coli
7.4.2.5 K274I change in selectivity Escherichia coli
7.4.2.5 L136R complete loss of uptake Escherichia coli
7.4.2.5 L137H no membrane localization Escherichia coli
7.4.2.5 L190V change in selectivity Escherichia coli
7.4.2.5 L324V change in selectivity Escherichia coli
7.4.2.5 L98R complete loss of uptake Escherichia coli
7.4.2.5 M154K change in selectivity Escherichia coli
7.4.2.5 M295K complete loss of uptake Escherichia coli
7.4.2.5 additional information screening of a library of mutants to identify amino acids critical for peptide transport and identification of 35 single point mutations that result in a full or partial loss of transport activity Escherichia coli
7.4.2.5 N196K partial loss of uptake Escherichia coli
7.4.2.5 N300I complete loss of uptake Escherichia coli
7.4.2.5 N300Y partial loss of uptake Escherichia coli
7.4.2.5 N306I partial loss of uptake Escherichia coli
7.4.2.5 P326Q partial loss of uptake Escherichia coli
7.4.2.5 Q320L complete loss of uptake Escherichia coli
7.4.2.5 R305C partial loss of uptake Escherichia coli
7.4.2.5 S59P complete loss of uptake Escherichia coli
7.4.2.5 T297A complete loss of uptake Escherichia coli
7.4.2.5 V252E change in selectivity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.4.2.5 alafosfalin
-
 Escherichia coli
7.4.2.5 L-Ala-L-Ala
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.5 Escherichia coli P77304
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.5 ATP + H2O + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/in
-
 Escherichia coli ADP + phosphate + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.5 YdgR
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.4.2.5 0.05
-
 alafosfalin mutant K274I, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.07
-
 alafosfalin wild-type, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.08
-
 L-Ala-L-Ala mutant M154K, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.2
-
 L-Ala-L-Ala mutant K274I, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.2
-
 L-Ala-L-Ala mutant L190V, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.21
-
 L-Ala-L-Ala mutant V252E, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.21
-
 L-Ala-L-Ala wild-type, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.25
-
 alafosfalin mutant M154K, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.28
-
 alafosfalin mutant V252E, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.31
-
 L-Ala-L-Ala mutant F197I, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.36
-
 L-Ala-L-Ala mutant L324V, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.38
-
 alafosfalin mutant L190V, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.45
-
 alafosfalin mutant F197I, pH 7.5, temperature not specified in the publication Escherichia coli
7.4.2.5 0.96
-
 alafosfalin mutant L324V, pH 7.5, temperature not specified in the publication Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.4.2.5 0.08
-
 mutant K274I, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 0.11
-
 wild-type, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 0.12
-
 mutant M154K, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.31
-
 mutant K274I, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.32
-
 mutant L190V, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.33
-
 mutant V252E, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.33
-
 wild-type, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.39
-
 mutant M154K, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 0.44
-
 mutant V252E, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 0.48
-
 mutant F197I, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.56
-
 mutant L324V, pH 7.5, temperature not specified in the publication Escherichia coli L-Ala-L-Ala
7.4.2.5 0.59
-
 mutant L190V, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 0.71
-
 mutant F197I, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin
7.4.2.5 1.5
-
 mutant L324V, pH 7.5, temperature not specified in the publication Escherichia coli alafosfalin