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Literature summary extracted from

  • Huang, X.; Kocabas, E.; Hernick, M.
    The activity and cofactor preferences of N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) change depending on environmental conditions (2011), J. Biol. Chem., 286, 20275-20282.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.103 34
-
 N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 36
-
 N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 41
-
 N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 53
-
 N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 54
-
 N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 56
-
 N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.103 Co2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
3.5.1.103 Fe2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
3.5.1.103 Mn2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
3.5.1.103 additional information enzyme has a strong preference for the Fe2+ cofactor under anaerobic conditions, regardless of the metal ion content of the medium. The preferred cofactor changes between Zn2+ and Fe2+ depending on the metal ion content of the medium when MshB is purified under aerobic conditions Mycolicibacterium smegmatis
3.5.1.103 Ni2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
3.5.1.103 Zn2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.103 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.103 N-acetyl-1D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside + H2O
-
 Mycolicibacterium smegmatis 1D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside + acetate
-
 ?
3.5.1.103 N-acetyl-D-glucosamine + H2O
-
 Mycolicibacterium smegmatis D-glucosamine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.103 MshB
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.103 0.11
-
 N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.13
-
 N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.27
-
 N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.38
-
 N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 1.1
-
 N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 2.1
-
 N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.103 0.0018
-
 N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.0036
-
 N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.0053
-
 N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.007
-
 N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.032
-
 N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis
3.5.1.103 0.052
-
 N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30°C Mycolicibacterium smegmatis