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Literature summary extracted from

  • Ohnuma, S.; Watanabe, M.; Nishino, T.
    Identification and characterization of geranylgeraniol kinase and geranylgeranyl phosphate kinase from the archaebacterium Sulfolobus acidocaldarius (1996), J. Biochem., 119, 541-547.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.B19 EDTA
-
 Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.B19 0.000027
-
 geranylgeraniol pH 7.0, 55°C, assay with membrane fraction Sulfolobus acidocaldarius
2.7.1.B19 0.65
-
 ATP pH 7.0, 55°C, assay with membrane fraction Sulfolobus acidocaldarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.B19 membrane
-
 Sulfolobus acidocaldarius 16020
-
2.7.4.B4 cytosol
-
 Sulfolobus acidocaldarius 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.B19 Co2+ 5 mM, activates. No activity in absence of cations Sulfolobus acidocaldarius
2.7.1.B19 Mg2+ 5 mM, activates. No activity in absence of cations Sulfolobus acidocaldarius
2.7.1.B19 Mn2+ 5 mM, activates. No activity in absence of cations Sulfolobus acidocaldarius
2.7.1.B19 Ni2+ 5 mM, activates. No activity in absence of cations Sulfolobus acidocaldarius
2.7.1.B19 Zn2+ 5 mM, activates. No activity in absence of cations Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.B19 ATP + geranylgeraniol Sulfolobus acidocaldarius all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation ADP + geranylgeranyl phosphate
-
 ?
2.7.4.B4 ATP + geranylgeranyl phosphate Sulfolobus acidocaldarius all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation ADP + geranylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.B19 Sulfolobus acidocaldarius
-
-
-
2.7.4.B4 Sulfolobus acidocaldarius
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.B19 ATP + (2E,6E)-farnesol 7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius ADP + (2E,6E)-farnesyl phosphate
-
 ?
2.7.1.B19 ATP + all-trans-hexaprenol 38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius ADP + all-trans-hexaprenyl phosphate
-
 ?
2.7.1.B19 ATP + all-trans-octaprenol 24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius ADP + all-trans-octaprenyl phosphate
-
 ?
2.7.1.B19 ATP + geranylgeraniol all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation Sulfolobus acidocaldarius ADP + geranylgeranyl phosphate
-
 ?
2.7.1.B19 ATP + geranylgeraniol all-trans-geranylgeraniol and ATP are the best substrates, activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes. No formation of geranylgeranyl diphosphate is observed Sulfolobus acidocaldarius ADP + geranylgeranyl phosphate
-
 ?
2.7.1.B19 CTP + geranylgeraniol 63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius CDP + geranylgeranyl phosphate
-
 ?
2.7.1.B19 GTP + geranylgeraniol 87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius GDP + geranylgeranyl phosphate
-
 ?
2.7.1.B19 additional information neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates Sulfolobus acidocaldarius ?
-
 ?
2.7.1.B19 UTP + geranylgeraniol 76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes Sulfolobus acidocaldarius UDP + geranylgeranyl phosphate
-
 ?
2.7.4.B4 ATP + geranylgeranyl phosphate
-
 Sulfolobus acidocaldarius ADP + geranylgeranyl diphosphate
-
 ?
2.7.4.B4 ATP + geranylgeranyl phosphate all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation Sulfolobus acidocaldarius ADP + geranylgeranyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.B19 GGOH kinase
-
 Sulfolobus acidocaldarius
2.7.4.B4 GGP kinase
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.B19 55
-
 assay at Sulfolobus acidocaldarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.B19 70 90 activity at 70% is about 40% of the activity at 90°C, no activity below 40°C Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.B19 80
-
 1 h, about 15% loss of activity Sulfolobus acidocaldarius
2.7.1.B19 95
-
 1 h, more than 90% loss of activity Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.B19 6.5 8.5
-
 Sulfolobus acidocaldarius