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Literature summary extracted from

  • Marco-Urrea, E.; Paul, S.; Khodaverdi, V.; Seifert, J.; von Bergen, M.; Kretzschmar, U.; Adrian, L.
    Identification and characterization of a Re-citrate synthase in Dehalococcoides strain CBDB1 (2011), J. Bacteriol., 193, 5171-5178.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.3 AMP about 120% activity at 0.15 mM Dehalococcoides sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.3 expressed in Escherichia coli BL21(DE3) cells Dehalococcoides sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.3 ATP about 40% residual activity at 5 mM Dehalococcoides sp.
2.3.3.3 citrate about 40% residual activity at 10 mM Dehalococcoides sp.
2.3.3.3 L-lysine about 80% residual activity at 0.5 mM Dehalococcoides sp.
2.3.3.3 additional information not influenced by 2-oxoglutarate Dehalococcoides sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.3 Mn2+ 0.2 mM Mn2+ is required for full activity Dehalococcoides sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.3 Dehalococcoides sp. P0DO96 designated as EC 2.3.3.14 but does not have any homocitrate synthase activity
-
2.3.3.3 Dehalococcoides sp. CBDB1 P0DO96 designated as EC 2.3.3.14 but does not have any homocitrate synthase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.3 Strep tag affinity column chromatography Dehalococcoides sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate
-
 Dehalococcoides sp. citrate + CoA
-
 ?
2.3.3.3 acetyl-CoA + H2O + oxaloacetate
-
 Dehalococcoides sp. CBDB1 citrate + CoA
-
 ?
2.3.3.3 additional information the protein does not have homocitrate synthase activity Dehalococcoides sp. ?
-
 ?
2.3.3.3 additional information the protein does not have homocitrate synthase activity Dehalococcoides sp. CBDB1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.3 cbdbA1708
-
 Dehalococcoides sp.
2.3.3.3 Re-citrate synthase
-
 Dehalococcoides sp.
2.3.3.3 Re-CS
-
 Dehalococcoides sp.