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Literature summary extracted from
- An alpha/beta-fold C-C bond hydrolase is involved in a central step of nicotine catabolism by Arthrobacter nicotinovorans (2005), J. Bacteriol., 187, 8516-8519.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.7.1.19 | expressed in Escherichia coli XL-1 Blue cells | Paenarthrobacter nicotinovorans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.19 | 0.006 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.19 | 43000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
| 3.7.1.19 | 43555 | - |
1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.19 | Paenarthrobacter nicotinovorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.19 | Ni-chelating Sepharose column chromatography | Paenarthrobacter nicotinovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.19 | 2,6-dihydroxypseudooxynicotine + H2O | - |
Paenarthrobacter nicotinovorans | 2,6-dihydroxypyridine + 4-methylaminobutanoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.19 | monomer | 1 * 43000, SDS-PAGE | Paenarthrobacter nicotinovorans |
| 3.7.1.19 | monomer | 1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.19 | 2,6-dihydroxypseudooxynicotine hydrolase | - |
Paenarthrobacter nicotinovorans |
| 3.7.1.19 | DHPONH | - |
Paenarthrobacter nicotinovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.19 | 792 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.19 | 7.5 | - |
- |
Paenarthrobacter nicotinovorans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.19 | 5 | 10 | - |
Paenarthrobacter nicotinovorans |
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Release 2023.1 (January 2023)
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