EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.2.6 | 0.09 | - |
2-oxo-4(E)-hexenedioate | D-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida | |
5.3.2.6 | 0.103 | - |
2-oxo-4(E)-hexenedioate | L-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.2.6 | 2-oxo-4(E)-hexenedioate | Pseudomonas putida | 1,3-allylic isomerization | 2-oxo-3(E)-hexenedioate | - |
? | |
5.3.2.6 | 2-oxo-4(E)-hexenedioate | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 1,3-allylic isomerization | 2-oxo-3(E)-hexenedioate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.2.6 | Pseudomonas putida | - |
- |
- |
5.3.2.6 | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.2.6 | 2-oxo-4(E)-hexenedioate | 1,3-allylic isomerization | Pseudomonas putida | 2-oxo-3(E)-hexenedioate | - |
? | |
5.3.2.6 | 2-oxo-4(E)-hexenedioate | 1,3-allylic isomerization | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-oxo-3(E)-hexenedioate | - |
? | |
5.3.2.6 | additional information | the achiral substrate 2-hydroxymuconate is processed with equal efficiency by either the D- or the L-enzyme, stereochemical analysis of the D-4OT-catalyzed reaction, overviewn | Pseudomonas putida | ? | - |
? | |
5.3.2.6 | additional information | the achiral substrate 2-hydroxymuconate is processed with equal efficiency by either the D- or the L-enzyme, stereochemical analysis of the D-4OT-catalyzed reaction, overviewn | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.2.6 | hexamer | the noncovalent structures of each 4OT enantiomer are similar, the predominant form of the enzyme is a hexameric complex of identical subunits | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.2.6 | 4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
5.3.2.6 | 4OT | - |
Pseudomonas putida |
5.3.2.6 | D-4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
5.3.2.6 | L-4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.2.6 | 30 | - |
assay at | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.2.6 | 2890 | - |
2-oxo-4(E)-hexenedioate | D-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida | |
5.3.2.6 | 2940 | - |
2-oxo-4(E)-hexenedioate | L-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.2.6 | 7.3 | - |
assay at | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.2.6 | metabolism | 4-oxalocrotonate tautomerase is part of a set of inducible enzymes that converts aromatic hydrocarbons to intermediates in the Krebs cycle | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.2.6 | 29000 | - |
2-oxo-4(E)-hexenedioate | L-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida | |
5.3.2.6 | 32000 | - |
2-oxo-4(E)-hexenedioate | D-4OT enantiomer, pH 7.3, 30°C | Pseudomonas putida |