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Literature summary extracted from

  • Kim, J.H.; Wang, R.; Lee, W.H.; Park, C.S.; Lee, S.; Yoo, S.H.
    One-pot synthesis of cycloamyloses from sucrose by dual enzyme treatment: combined reaction of amylosucrase and 4-alpha-glucanotransferase (2011), J. Agric. Food Chem., 59, 5044-5051.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.25 synthesis development of an efficient biocatalytic production process of cycloamyloses directly from sucrose by use of Synechocystis sp. 4-alpha-glucanotransferase and Neisseria polysaccharea amylosucrase. From one-pot synthesis, the maximum cycloamylose yield of 9.6%, w/w with 0.3 M sucrose is achieved with 10 units/ml of amylosucrase and 0.1 unit/ml of 4-alpha-glucanotransferase at 40°C for a 3 h reaction in a simultaneous dual enzyme reaction mode. The size of linear alpha-(1,4)-glucan is positively related to the cycloamylose productivity by 4-alpha-glucanotransferase in a hyperbolic manner. The dual enzyme reaction converts sucrose directly to cycloamyloses via in situ transient linear alpha-(1,4)-glucan as an soluble intermediate Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n Neisseria polysaccharea
-
 D-fructose + [(1->4)-alpha-D-glucosyl]n+1 linear alpha-(1,4)-glucans ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Neisseria polysaccharea
-
-
-
2.4.1.25 Synechocystis sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 additional information synthesis of cycloamyloses from sucrose by dual enzyme treatment via combined reaction of amylosucrase and 4-alpha-glucanotransferase from Synechocystis sp., EC 2.4.1.25, overview Neisseria polysaccharea ?
-
 ?
2.4.1.4 sucrose + amylose
-
 Neisseria polysaccharea D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + maltose
-
 Neisseria polysaccharea D-fructose + maltotriose
-
 ?
2.4.1.4 sucrose + starch
-
 Neisseria polysaccharea D-fructose + ?
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Neisseria polysaccharea D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Neisseria polysaccharea D-fructose + [(1->4)-alpha-D-glucosyl]n+1 linear alpha-(1,4)-glucans ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4alphaGTase
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 35
-
-
 Neisseria polysaccharea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
 assay at Neisseria polysaccharea