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Literature summary extracted from

  • Feltrer, R.; Alvarez-Rodriguez, M.L.; Barreiro, C.; Godio, R.P.; Coque, J.J.
    Characterization of a novel 2,4,6-trichlorophenol-inducible gene encoding chlorophenol O-methyltransferase from Trichoderma longibrachiatum responsible for the formation of chloroanisoles and detoxification of chlorophenols (2010), Fungal Genet. Biol., 47, 458-467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.136 expressed in Escherichia coli BL21(DE3) cells Trichoderma longibrachiatum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.136 additional information partial silencing of cmt1 gene results in a 48.9% decrease of enzyme activity levels in a Trichoderma longibrachiatum At37 transformant strain by comparison with the wild type Trichoderma longibrachiatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.136 52400
-
 x * 52400, deduced from amino acid sequence Trichoderma longibrachiatum
2.1.1.136 65400
-
 x * 65400, fusion protein SUMO-CMT1, SDS-PAGE Trichoderma longibrachiatum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.136 Trichoderma longibrachiatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.136 DEAE column chromatography. hydroxyapatite column chromatography, Resource Q column chromatography, ultrafiltration, and Superdex 200 gel filtration Trichoderma longibrachiatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.136 mycelium
-
 Trichoderma longibrachiatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.136 S-adenosyl-L-methionine + 2,4,6-trichlorophenol
-
 Trichoderma longibrachiatum S-adenosyl-L-homocysteine + 2,4,6-trichloroanisole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.136 ? x * 52400, deduced from amino acid sequence Trichoderma longibrachiatum
2.1.1.136 ? x * 65400, fusion protein SUMO-CMT1, SDS-PAGE Trichoderma longibrachiatum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.136 CMT1 formerly named CPOMT Trichoderma longibrachiatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.136 Trichoderma longibrachiatum deduced from amino acid sequence
-
 5.9

Expression

EC Number Organism Comment Expression
2.1.1.136 Trichoderma longibrachiatum the expression of the enzyme is induced at transcriptional level by exposure of fungal mycelia to 0.01 mg/ml 2,4,6-trichlorophenol up

General Information

EC Number General Information Comment Organism
2.1.1.136 physiological function the enzyme is responsible for the detoxification of chlorophenolic pesticides by O-methylation which results in the formation of chloroanisoles Trichoderma longibrachiatum