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Literature summary extracted from

  • Park, J.; Yun, J.; Lim, J.A.; Kang, D.H.; Ryu, S.
    Characterization of an endolysin, LysBPS13, from a Bacillus cereus bacteriophage (2012), FEMS Microbiol. Lett., 332, 76-83.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.28 NaCl the highest lytic activity is observed in the presences of 250 mM NaCl Bacillus cereus phage

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 expressed in Escherichia coli BL21 Star(DE3) cells Bacillus cereus phage

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.28 CTAB almost complete inhibition at 0.1 (v/v) Bacillus cereus phage
3.5.1.28 additional information the activity of the enzyme is not inhibited by 300 mM EDTA or CHAPS, Triton X-100 and Tween-20 (0.1% (v/v) each) Bacillus cereus phage
3.5.1.28 SDS almost complete inhibition at 0.1 (v/v) Bacillus cereus phage

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.28 additional information the enzyme needs no metal ions for its lytic activity, 1 mM MgCl2, CaCl2, ZnCl2, or MnCl2 does not affect lytic activity Bacillus cereus phage
3.5.1.28 Zn2+ the enzyme contains three zinc-binding sites Bacillus cereus phage

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.28 35000
-
 x * 35000, SDS-PAGE Bacillus cereus phage

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 peptidoglycan + H2O Bacillus cereus phage
-
 ?
-
 ?
3.5.1.28 peptidoglycan + H2O Bacillus cereus phage BPS13
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.28 Triton X-100 about 130% activity in the presence of 0.1 (v/v) Triton X-100 Bacillus cereus phage
3.5.1.28 Tween 20 about 115% activity in the presence of 0.1 (v/v) Tween 20 Bacillus cereus phage

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Bacillus cereus phage
-
-
-
3.5.1.28 Bacillus cereus phage BPS13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 Ni-NTA resin column chromatography Bacillus cereus phage

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O
-
 Bacillus cereus phage N-acetylmuramate + L-alanine
-
 ?
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O
-
 Bacillus cereus phage BPS13 N-acetylmuramate + L-alanine
-
 ?
3.5.1.28 peptidoglycan + H2O
-
 Bacillus cereus phage ?
-
 ?
3.5.1.28 peptidoglycan + H2O
-
 Bacillus cereus phage BPS13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.28 ? x * 35000, SDS-PAGE Bacillus cereus phage

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 endolysin
-
 Bacillus cereus phage
3.5.1.28 LysBPS13
-
 Bacillus cereus phage
3.5.1.28 N-acetylmuramyl-L-alanine amidase
-
 Bacillus cereus phage

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.28 42 45
-
 Bacillus cereus phage

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.28 35 55 about 50% activity at 35 and 55°C Bacillus cereus phage

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.28 4 100 the enzyme demonstrates remarkable thermostability in the presence of 30% (v/v) glycerol, and it retains its lytic activity after incubation between 4 and 100°C for 30 min Bacillus cereus phage

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 9.5
-
-
 Bacillus cereus phage

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.28 7.5 10.5 lytic activity significantly decreases at above pH 10.5 and below pH 7.5 Bacillus cereus phage

General Information

EC Number General Information Comment Organism
3.5.1.28 physiological function endolysin LysBPS13 demonstrates high lytic activity against Bacillus cereus Bacillus cereus phage