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Literature summary extracted from

  • Ando, S.; Ishikawa, K.; Ishida, H.; Kawarabayasi, Y.; Kikuchi, H.; Kosugi, Y.
    Thermostable aminopeptidase from Pyrococcus horikoshii (1999), FEBS Lett., 447, 25-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.B4 expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.B4 10.8
-
 Ala-Ala-Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 13.2
-
 Ala-Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 18.2
-
 Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 39.9
-
 Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.B4 Ca2+ the enzyme is chelated with one calcium ion per monomer of the protein. Removal of Ca2+ by dialysis for 12 h in 50 mM EDTA does not effect the enzyme activity, suggesting that calcium has no effect on the activity. Replacement of calcium with zinc also shows no effect on the activity Pyrococcus horikoshii
3.4.11.B4 Co2+ activity is dependent on Co2+. 6fold increase in activity in presence of 0.5 mM Co2+ Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.B4 36900
-
 x * 36900, SDS-PAGE Pyrococcus horikoshii
3.4.11.B4 440000
-
 gel filtration, HPLC Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.B4 Pyrococcus horikoshii O58255
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.B4
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.B4 Ala-Ala + H2O
-
 Pyrococcus horikoshii L-alanine + L-alanine
-
 ?
3.4.11.B4 Ala-Ala-Ala + H2O the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala Pyrococcus horikoshii Ala-Ala + L-alanine
-
 ?
3.4.11.B4 Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii Ala-Ala-Ala + L-alanine
-
 ?
3.4.11.B4 Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii Ala-Ala-Ala-Ala + L-alanine
-
 ?
3.4.11.B4 additional information no activity with Ala-Pro-Ala Pyrococcus horikoshii ?
-
 ?
3.4.11.B4 N-acetyl-Ala-Ala-Ala + H2O the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala Pyrococcus horikoshii Ala-Ala + N-acetyl-L-alanine
-
 ?
3.4.11.B4 N-acetyl-L-alanyl 4-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-L-alanine + 4-nitroaniline
-
 ?
3.4.11.B4 N-acetyl-L-leucyl 4-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-L-leucine + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.B4 oligomer x * 36900, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.4.11.B4 PH0519
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.B4 95
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.B4 85 98 85°C: about 35% of maximal activity, 98°C: about 85% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.B4 90
-
 2 h, about 20% loss of activity Pyrococcus horikoshii
3.4.11.B4 95
-
 about 15% loss of activity after 1 h, about 70% loss of activity after 2 h Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.B4 0.022
-
 N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 0.116
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 0.18
-
 Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
3.4.11.B4 8.71
-
 Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.B4 7.5
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.B4 7 8.3 pH 6.5: about 20% of maximal activity, pH 7.0: about 90% of maximal activity, pH 8.3: about 50% of maximal activity Pyrococcus horikoshii