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Literature summary extracted from

  • Kumar, R.; Mukhopadhyay, A.K.; Rao, D.N.
    Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand (2010), FEBS J., 277, 1666-1683.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli BL21 (DE3) pLysS cells Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 F195S the mutant is not able to bind to the S-adenosyl-L-methionine as effectively as the wild type enzyme and is catalytically inactive Helicobacter pylori
2.1.1.72 Y32L the mutant binds to S-adenosyl-L-methionine as efficiently as wild type enzyme but is catalytically inactive Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 0.0199
-
 DNA adenine in 10 mM Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, at 37°C Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 28000
-
 gel filtration Helicobacter pylori
2.1.1.72 32000
-
 1 * 32000, His-tagged enzyme, SDS-PAGE Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni-NTA column chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the enzyme recognizes and methylates GAGG (100% activity, Km 0.00522 mM), GGAG (about 48% activity, Km 0.017 mM) and GAAG (about 30% activity, Km 0.013 mM), but does not methylate GTGG, GmAmAG or GAGA Helicobacter pylori ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 monomer 1 * 32000, His-tagged enzyme, SDS-PAGE Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
-
 Helicobacter pylori
2.1.1.72 HP0050 methyltransferase
-
 Helicobacter pylori
2.1.1.72 N6 adenine methyltransferase
-
 Helicobacter pylori
2.1.1.72 N6 adenine MTase
-
 Helicobacter pylori

General Information

EC Number General Information Comment Organism
2.1.1.72 malfunction inactivation of the enzyme results in poor growth Helicobacter pylori