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Literature summary extracted from
- Purification, characterization, and effect of thiol compounds on activity of the Erwinia carotovora L-asparaginase (2010), Enzyme Res., 2010, 165878.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | 2-mercaptoethanol | activates 112% at 1 mM | Pectobacterium carotovorum |  |
| 3.5.1.1 | EDTA | activates 117% at 1 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | GSH | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation, activates 136% at 1 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | L-cysteine | activates 148% at 1 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | L-cystine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
| 3.5.1.1 | L-histidine | activates 148% at 1 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | L-methionine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
| 3.5.1.1 | N-acetyl-L-cysteine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
| 3.5.1.1 | SDS | 78% inhibition at 3.0 mM, 119% activation at 2.0 mM | Pectobacterium carotovorum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | 4-chloromercuribenzoate | complete inhibition at 0.5 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Ca2+ | 22% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Cd2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Cu2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | dithiothreitol | 14% inhibition at 0.5 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Fe3+ | 53% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | H2O2 | 76% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Hg2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | iodoacetamide | complete inhibition at 0.5 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Mg2+ | 76% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Mn2+ | 29% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Ni2+ | 49% inhibition at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | SDS | 78% inhibition at 3.0 mM, 119% activation at 1.0 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Zn2+ | 94% inhibition at 1.0 mM | Pectobacterium carotovorum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | additional information | - |
additional information | Lineweaver-Burk and Michaelis-Menten kinetic analysis of activity, overview. Thiol compounds reduce the Km and increase the Vmax vlaues | Pectobacterium carotovorum | |
| 3.5.1.1 | 0.098 | - |
L-asparagine | pH 8.5, 37°C | Pectobacterium carotovorum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.1 | K+ | activates 133% at 1 mM | Pectobacterium carotovorum | |
| 3.5.1.1 | Na+ | activates 117% at 1 mM | Pectobacterium carotovorum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 37000 | - |
x * 37000, SDS-PAGE | Pectobacterium carotovorum |
| 3.5.1.1 | 150000 | - |
gel filtration | Pectobacterium carotovorum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | Pectobacterium carotovorum | - |
L-aspartate + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Pectobacterium carotovorum | - |
isolated from soil after screening | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | native enzyme 752.9fold by ammonium sulfate fractionation, gel filtration, cation and anion exchange chromatography | Pectobacterium carotovorum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 1034 | - |
purified enzyme, pH 8.5, 37°C | Pectobacterium carotovorum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | L-asparagine + H2O | specific for | Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | additional information | the hydrolysis efficiency of asparagine is at least 11925fold higher than that of L-glutamine | Pectobacterium carotovorum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | oligomer | x * 37000, SDS-PAGE | Pectobacterium carotovorum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | L-asparaginase | - |
Pectobacterium carotovorum |
| 3.5.1.1 | L-asparagine amino hydrolase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 37 | - |
assay at | Pectobacterium carotovorum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 8.5 | - |
assay at | Pectobacterium carotovorum |
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