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Literature summary extracted from

  • Carpenter, M.A.; Rajagurubandara, E.; Wijesinghe, P.; Bhagwat, A.S.
    Determinants of sequence-specificity within human AID and APOBEC3G (2010), DNA Repair, 9, 579-587.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.B9 the carboxyl-terminal catalytic domain is expressed in Escherichia coli BL-21 DE3 codon+RIL cells Homo sapiens
3.5.4.38
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.38 additional information construction of segment swaps between activation-induced deaminase (AID) and APOBEC3G and determination of their mutational sequence specificity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.B9 Zn2+ the active site of the carboxy-terminal domain contains a Zn2+ ion Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O Homo sapiens the enzyme strongly prefers cytosines in a run of C's usually targeting the last base in the run. The enzyme readily converts the third cytosine in CCC to uracil but does not convert the first or the second cytosine in the sequence at detectable levels uracil in single-stranded viral DNA + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens Q9HC16
-
-
3.5.4.38 Homo sapiens Q9GZX7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.B9 glutathione Sepharose column chromatography and MonoQ column chromatography Homo sapiens
3.5.4.38 activation-induced deaminase(AID)/APOBEC3G hybrid proteins Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O the enzyme strongly prefers cytosines in a run of C's usually targeting the last base in the run. The enzyme readily converts the third cytosine in CCC to uracil but does not convert the first or the second cytosine in the sequence at detectable levels Homo sapiens uracil in single-stranded viral DNA + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 A3G
-
 Homo sapiens
3.5.4.B9 APOBEC3G
-
 Homo sapiens
3.5.4.38 activation-induced cytidine deaminase
-
 Homo sapiens
3.5.4.38 AID
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.38 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.38 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.4.B9 physiological function the enzyme causes C to T mutations in the cDNA copy of the HIV-1 genome Homo sapiens