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Literature summary extracted from

  • Lang, Z.F.; Shen, J.J.; Cai, S.; Zhang, J.; He, J.; Li, S.P.
    Expression, characterization, and site-directed mutation of a multiple herbicide-resistant acetohydroxyacid synthase (rAHAS) from Pseudomonas sp. Lm10 (2011), Curr. Microbiol., 63, 145-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6
-
 Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 additional information cloning of a herbicide-resistant acetohydroxyacid synthase gene from Pseudomonas sp. Lm10. Sequence analysis shows that the regulatory subunit of the resisitant enzyme is identical to that of Pseudomonas putida KT2440, whereas six mutations are found in the catalytic subunit, i.e. resistant AHAS/sensitive AHAS: H134N, A135P, S136T, I210V, F264Y, and S486W Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 flumetsulam herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 6.5fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
2.2.1.6 imazethapyr herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 12.6fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
2.2.1.6 L-valine
-
 Pseudomonas sp.
2.2.1.6 metsulfuron-methyl herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 56fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.
2.2.1.6 pyriminobac-methyl herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 9.2fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 17.5
-
 pyruvate pH 7.5, 37°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ Km value 0.00012 mM Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 62000
-
 x * 62000, SDS-PAGE, catalytic subunit Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Pseudomonas sp. G0ZSE6 catalytic subunit
-
2.2.1.6 Pseudomonas sp. Lm10 G0ZSE6 catalytic subunit
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 13.5
-
 pH 7.5, 37°C Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 -70°C, 3 weeks, loss of 5% of activity Pseudomonas sp.
2.2.1.6 4°C, 3 weeks, loss of 10% of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Pseudomonas sp. 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Pseudomonas sp. Lm10 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 62000, SDS-PAGE, catalytic subunit Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD Km value 0.00033 mM Pseudomonas sp.
2.2.1.6 thiamine diphosphate Km value 0.0092 mM Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 0.036
-
 flumetsulam pH 7.5, 37°C Pseudomonas sp.
2.2.1.6 0.065
-
 pyriminobac-methyl pH 7.5, 37°C Pseudomonas sp.
2.2.1.6 0.105
-
 L-valine pH 7.5, 37°C Pseudomonas sp.
2.2.1.6 0.121
-
 imazethapyr pH 7.5, 37°C Pseudomonas sp.
2.2.1.6 0.201
-
 metsulfuron-methyl pH 7.5, 37°C Pseudomonas sp.