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Literature summary extracted from

  • Rale, M.; Schneider, S.; Sprenger, G.A.; Samland, A.K.; Fessner, W.D.
    Broadening deoxysugar glycodiversity: natural and engineered transaldolases unlock a complementary substrate space (2011), Chemistry, 17, 2623-2632.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.2 F178E mutant is able to catalyze aldol reactions and readily accepts hihydroxypropanone and hydroxypropanone Escherichia coli
2.2.1.2 F178E/S176A improved acceptance of substrate hydroxypropanone compared with mutant F178E Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.2 D-Lactaldehyde
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 1.9
-
 glyceraldehyde 3-phosphate wild-type, pH 8.5, 30°C Escherichia coli
2.2.1.2 2.4
-
 glyceraldehyde 3-phosphate mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 59
-
 D-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 120
-
 DL-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 dihydroxypropanone + D-glyceraldehyde
-
 Escherichia coli D-fructose
-
 ?
2.2.1.2 dihydroxypropanone + DL-glyceraldehyde
-
 Escherichia coli D-fructose
-
 ?
2.2.1.2 dihydroxypropanone + glyceraldehyde 3-phosphate
-
 Escherichia coli D-fructose 6-phosphate
-
 ?
2.2.1.2 additional information enzyme catalyzes a stereospecifc carboligation resulting in D-threo configuration of the product. Donor substrates require a 1-hydroxy-2-alkanone moiety. No substrates: propanone, butanone Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.2 0.53
-
 D-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 0.54
-
 DL-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 12
-
 glyceraldehyde 3-phosphate mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 13
-
 glyceraldehyde 3-phosphate wild-type, pH 8.5, 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.2 4.3
-
 DL-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 9
-
 D-glyceraldehyde mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 5000
-
 glyceraldehyde 3-phosphate mutant F178, pH 8.5, 30°C Escherichia coli
2.2.1.2 6600
-
 glyceraldehyde 3-phosphate wild-type, pH 8.5, 30°C Escherichia coli