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Literature summary extracted from

  • Llewellyn, N.M.; Li, Y.; Spencer, J.B.
    Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain (2007), Chem. Biol., 14, 379-386.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.19 expressed in Escherichia coli BL21(DE3) cells Niallia circulans
4.3.2.6 expressed in Escherichia coli BL21(DE3) cells Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.19 35900
-
 x * 35900, calculated from amino acid sequence Niallia circulans
4.3.2.6 18100
-
 1 * 18100, calculated from amino acid sequence Niallia circulans
4.3.2.6 20093
-
 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.19 Niallia circulans
-
-
-
2.3.2.19 Niallia circulans NR3312
-
-
-
4.3.2.6 Niallia circulans
-
-
-
4.3.2.6 Niallia circulans NR3312
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.6 Ni2+-NTA affinity column chromatography Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl -[BtrI acyl-carrier protein] + neomycin
-
 Niallia circulans gamma-L-glutamyl-neomycin + BtrI acyl-carrier protein
-
 ?
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl -[BtrI acyl-carrier protein] + neomycin
-
 Niallia circulans NR3312 gamma-L-glutamyl-neomycin + BtrI acyl-carrier protein
-
 ?
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl-[BtrI acyl-carrier protein] + paromomycin
-
 Niallia circulans gamma-L-glutamyl-paromomycin + BtrI acyl-carrier protein
-
 ?
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl-[BtrI acyl-carrier protein] + paromomycin
-
 Niallia circulans NR3312 gamma-L-glutamyl-paromomycin + BtrI acyl-carrier protein
-
 ?
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl-[BtrI acyl-carrier protein] + ribostamycin
-
 Niallia circulans gamma-L-glutamyl-butirosin B + BtrI acyl-carrier protein
-
 ?
2.3.2.19 4-(gamma-L-glutamylamino)-(S)-2-hydroxylbutanoyl-[BtrI acyl-carrier protein] + ribostamycin
-
 Niallia circulans NR3312 gamma-L-glutamyl-butirosin B + BtrI acyl-carrier protein
-
 ?
2.3.2.19 additional information the enzyme does not accept gentamicin C or kanamycin as substrates Niallia circulans ?
-
 ?
2.3.2.19 additional information the enzyme does not accept gentamicin C or kanamycin as substrates Niallia circulans NR3312 ?
-
 ?
4.3.2.6 gamma-L-glutamyl-butirosin B
-
 Niallia circulans butirosin B + 5-oxoproline
-
 ?
4.3.2.6 gamma-L-glutamyl-butirosin B
-
 Niallia circulans NR3312 butirosin B + 5-oxoproline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.19 ? x * 35900, calculated from amino acid sequence Niallia circulans
2.3.2.19 ? x * 37916. His-tagged enzyme, LC-electrospray ionization mass spectrometry Niallia circulans
4.3.2.6 monomer 1 * 18100, calculated from amino acid sequence Niallia circulans
4.3.2.6 monomer 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
2.3.2.19 ACP:aminoglycoside acyltransferase
-
 Niallia circulans
2.3.2.19 btrH
-
 Niallia circulans
4.3.2.6 btrG
-
 Niallia circulans
4.3.2.6 gamma-glutamyl cyclotransferase
-
 Niallia circulans
4.3.2.6 GGC
-
 Niallia circulans

General Information

EC Number General Information Comment Organism
4.3.2.6 metabolism the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin Niallia circulans