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Literature summary extracted from

  • Rose, N.L.; Zheng, R.B.; Pearcey, J.; Zhou, R.; Completo, G.C.; Lowary, T.L.
    Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase (2008), Carbohydr. Res., 343, 2130-2139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.288 expressed in Escherichia coli Rosetta (DE3) Quarters cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.288 0.0038
-
 UDP-alpha-D-galactofuranose apparent value, spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.288 73500
-
 x * 73500, His-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.288 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.288 Ni2+-NTA agarose column chromatography and Sephacryl S-100 gel filtration Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
2.4.1.288 -80°C, purified enzyme with 20% (v/v) glycerol, four months, no significant loss of activity Mycobacterium tuberculosis
2.4.1.288 4°C, purified enzyme with 20% (v/v) glycerol, 2 weeks, no significant loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol the enzyme adds the third and subsequent alpha-D-galactofuranosyl residues to the growing galactan chain Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3
-
 Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.288 ? x * 73500, His-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.288 galactofuranosyltransferase
-
 Mycobacterium tuberculosis
2.4.1.288 GlfT2
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.288 7.2
-
 UDP-alpha-D-galactofuranose spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C Mycobacterium tuberculosis