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Literature summary extracted from

  • Soontharapirakkul, K.; Incharoensakdi, A.
    Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism (2010), BMC Biochem., 11, 30.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.3 ATP ATPase activity increases as ATP increased and the activity is saturated at about 6 mM ATP Aphanothece halophytica
7.2.2.3 carbonyl cyanide m-chlorophenyl hydrazine
-
 Aphanothece halophytica
7.2.2.3 nitrate
-
 Aphanothece halophytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.3 additional information not inhibited by 0.2 mM amiloride, 0.01 mM orthovanadate, 0.5 mM NEM, 50 mM KNO3, 20 mM KSCN, 1 mM CCP, and 1 mM ouabain Aphanothece halophytica
7.2.2.3 N,N’-dicyclohexylcarbodiimide 78% residual activity at 1 mM in the presence of 10 mM NaCl. Inhibition in the absence of NaCl occurs after 20 min yielding 80 and 70% inhibition at pH 7.6 and pH 9.0, respectively Aphanothece halophytica
7.2.2.3 Sodium azide 64% residual activity at 5 mM Aphanothece halophytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.3 1.2
-
 ATP apparent value, at pH 7.6, temperature not specified in the publication Aphanothece halophytica
7.2.2.3 2
-
 Na+/in apparent value, at pH 7.6, temperature not specified in the publication Aphanothece halophytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.3 membrane proteoliposome Aphanothece halophytica 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.3 Mg2+ maximal ATPase activity is observed at 5 mM Mg2+ Aphanothece halophytica
7.2.2.3 additional information K+, Li+, and Ca2+ have no stimulatory effect on enzyme activity Aphanothece halophytica
7.2.2.3 Na+ the enzyme is stimulated about 9fold at pH 7.6 and about 11fold at both pH 9.0 and 11.0 with 10 mM NaCl Aphanothece halophytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.3 6500
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 16500
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 22000
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 25000
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 35000
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 52000
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica
7.2.2.3 56000
-
 x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.3 Aphanothece halophytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.3 PEG 6000 precipitation and Superose 6 gel filtration Aphanothece halophytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.3 0.0266
-
 crude enzyme from membrane vesicles, at pH 7.6, temperature not specified in the publication Aphanothece halophytica
7.2.2.3 0.4667
-
 after 17.5fold purification, at pH 7.6, temperature not specified in the publication Aphanothece halophytica

Storage Stability

EC Number Storage Stability Organism
7.2.2.3 -20°C, purified enzyme, 1 month, the enzyme remains stable Aphanothece halophytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.3 ATP + H2O + Na+/in the purified enzyme catalyzes the hydrolysis of ATP in the presence of Na+ but not K+, Li+ and Ca2+ Aphanothece halophytica ADP + phosphate + Na+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.3 ? x * 56000 + x * 52000 + x * 35000 + x * 25000 + x * 22000 + x * 16500 + x * 6500, SDS-PAGE Aphanothece halophytica

Synonyms

EC Number Synonyms Comment Organism
7.2.2.3 Na+-stimulated ATPase
-
 Aphanothece halophytica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
7.2.2.3 6 11 when the pH is increased from pH 6.0 to pH 9.0, the enzyme activity is increased progressively. Increasing the pH further to pH 11.0 results in a slightly declined enzyme activity Aphanothece halophytica

Expression

EC Number Organism Comment Expression
7.2.2.3 Aphanothece halophytica cells grown at 2 M NaCl show higher enzyme activity than those grown at 0.5 M NaCl. ATPase activity in is slightly increased upon increasing the pH from 5.0 to 7.6 up

General Information

EC Number General Information Comment Organism
7.2.2.3 physiological function the enzyme functions as an electrogenic Na+ pump which transports only Na+ upon hydrolysis of ATP. Na+- and ATP-dependent H+ efflux from proteoliposomes, is driven by the electric potential generated by Na+-stimulated ATPase Aphanothece halophytica