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Literature summary extracted from
- The N-terminal region is important for the nuclease activity and thermostability of the flap endonuclease-1 from Sulfolobus tokodaii (2007), Biosci. Biotechnol. Biochem., 71, 855-865.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.99.B1 | expression in Escherichi coli | Sulfurisphaera tokodaii |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.99.B1 | Mg2+ | enzyme form StolL-FEN-1 exhibits activity in the concentration range 0.5-10 mM | Sulfurisphaera tokodaii |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.99.B1 | 41000 | - |
x * 41000, enzyme form StoL-FEN-1, SDS-PAGE | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.99.B1 | Sulfurisphaera tokodaii | Q976H6 | - |
- |
| 3.1.99.B1 | Sulfurisphaera tokodaii 7 | Q976H6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.99.B1 | - |
Sulfurisphaera tokodaii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.99.B1 | -20°C, in 50% glycerol solution | Sulfurisphaera tokodaii |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.99.B1 | ? | x * 41000, enzyme form StoL-FEN-1, SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.99.B1 | FEN-1 | - |
Sulfurisphaera tokodaii |
| 3.1.99.B1 | FEN-1 | two types of recombinant flap endonuclease-1: one of the two FEN-1 proteins is a product of the gene with AUG as the translational start codon (StoS-FEN-1). The other is a product of the gene with a new AUG start codon (StoL-FEN-1), which is inserted at 153 bases upstream of the original AUG codon. StoL-FEN-1 showed activity and thermostability, StoS-FEN-1 shows neither activity nor thermostability | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.99.B1 | additional information | - |
the pre-ATG region is essentail for activity and thermostability of the enzyme form StoFEN-1 | Sulfurisphaera tokodaii |
| 3.1.99.B1 | 80 | - |
15 min, stable | Sulfurisphaera tokodaii |
| 3.1.99.B1 | 100 | - |
complete inactivation | Sulfurisphaera tokodaii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.99.B1 | 5 | 9 | enzyme form StolL-FEN-1 exhibits activity in the pH-range 5-9 | Sulfurisphaera tokodaii |
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Release 2023.1 (January 2023)
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