Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carrillo, D.R.; Parthier, C.; Jaenckel, N.; Grandke, J.; Stelter, M.; Schilling, S.; Boehme, M.; Neumann, P.; Wolf, R.; Demuth, H.U.; Stubbs, M.T.; Rahfeld, J.U.
    Kinetic and structural characterization of bacterial glutaminyl cyclases from Zymomonas mobilis and Myxococcus xanthus (2010), Biol. Chem., 391, 1419-1428.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5 expressed in Escherichia coli BL21(DE3) cells Zymomonas mobilis
2.3.2.5 expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.5 hanging drop vapor diffusion method, using 0.1 M MES and 30% (w/v) PEG-4000 Myxococcus xanthus
2.3.2.5 hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate and 12% (w/v) PEG-4000 Zymomonas mobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.5 E48Q the mutant’s pH optimum is shifted towards higher pH values Zymomonas mobilis
2.3.2.5 Q46E the mutant’s pH optimum is shifted towards lower pH values, although the activity towards L-glutamine-2-naphtylamine is some 1000fold lower than the wild type Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.5 0.05
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.1
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.1
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.1
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.2
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.3
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.4
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.4
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.5
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.7
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.8
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 0.9
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 1
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 1
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 1.1
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 1.1
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 1.6
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 1.9
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 2.1
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 2.4
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 2.5
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 2.7
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 2.9
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 2.9
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 3.5
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 3.8
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 4.2
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 5.6
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 6.5
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 8.2
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 20.4
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 24.4
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.5 29900
-
 x * 29900, MALDI-TOF mass spectrometry Zymomonas mobilis
2.3.2.5 30000
-
 x * 30000, SDS-PAGE Zymomonas mobilis
2.3.2.5 30000
-
 x * 30000, SDS-PAGE Myxococcus xanthus
2.3.2.5 31100
-
 x * 31100, MALDI-TOF mass spectrometry Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Myxococcus xanthus Q1DDS6
-
-
2.3.2.5 Zymomonas mobilis Q5NLA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5 Q-Sepharose column chromatography Zymomonas mobilis
2.3.2.5 Q-Sepharose column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 L-Gln-2-naphthylamide
-
 Zymomonas mobilis 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
2.3.2.5 L-Gln-2-naphthylamide
-
 Myxococcus xanthus 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
2.3.2.5 L-Gln-7-amido-4-methylcoumarin
-
 Zymomonas mobilis 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 L-Gln-7-amido-4-methylcoumarin
-
 Myxococcus xanthus 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 L-Gln-amide
-
 Zymomonas mobilis 5-oxoprolyl-amide + NH3
-
 ?
2.3.2.5 L-Gln-amide
-
 Myxococcus xanthus 5-oxoprolyl-amide + NH3
-
 ?
2.3.2.5 L-Gln-Gly
-
 Zymomonas mobilis 5-oxoprolylglycine + NH3
-
 ?
2.3.2.5 L-Gln-L-Gln
-
 Zymomonas mobilis 5-oxoprolyl-L-Gln + NH3
-
 ?
2.3.2.5 L-Gln-L-Gln
-
 Myxococcus xanthus 5-oxoprolyl-L-Gln + NH3
-
 ?
2.3.2.5 L-Gln-L-Glu
-
 Zymomonas mobilis 5-oxoprolyl-L-Glu + NH3
-
 ?
2.3.2.5 L-Gln-L-Glu
-
 Myxococcus xanthus 5-oxoprolyl-L-Glu + NH3
-
 ?
2.3.2.5 L-Gln-tert-butyl ester
-
 Zymomonas mobilis 5-oxoprolyl-tert-butyl ester + NH3
-
 ?
2.3.2.5 L-Gln-tert-butyl ester
-
 Myxococcus xanthus 5-oxoprolyl-tert-butyl ester + NH3
-
 ?
2.3.2.5 additional information the enzyme shows no activity towards L-Gln-Gly Myxococcus xanthus ?
-
 ?
2.3.2.5 additional information the enzyme shows no activity towards QAAR Zymomonas mobilis ?
-
 ?
2.3.2.5 QAAE
-
 Zymomonas mobilis 5-oxoprolyl-AAE + NH3
-
 ?
2.3.2.5 QAAE
-
 Myxococcus xanthus 5-oxoprolyl-AAE + NH3
-
 ?
2.3.2.5 QAAF
-
 Zymomonas mobilis 5-oxoprolyl-AAF + NH3
-
 ?
2.3.2.5 QAAF
-
 Myxococcus xanthus 5-oxoprolyl-AAF + NH3
-
 ?
2.3.2.5 QAAR
-
 Myxococcus xanthus 5-oxoprolyl-AAR + NH3
-
 ?
2.3.2.5 QAEA
-
 Zymomonas mobilis 5-oxoprolyl-AEA + NH3
-
 ?
2.3.2.5 QAEA
-
 Myxococcus xanthus 5-oxoprolyl-AEA + NH3
-
 ?
2.3.2.5 QAFA
-
 Zymomonas mobilis 5-oxoprolyl-AFA + NH3
-
 ?
2.3.2.5 QAFA
-
 Myxococcus xanthus 5-oxoprolyl-AFA + NH3
-
 ?
2.3.2.5 QARA
-
 Zymomonas mobilis 5-oxoprolyl-ARA + NH3
-
 ?
2.3.2.5 QARA
-
 Myxococcus xanthus 5-oxoprolyl-ARA + NH3
-
 ?
2.3.2.5 QEAA
-
 Zymomonas mobilis 5-oxoprolyl-EAA + NH3
-
 ?
2.3.2.5 QEAA
-
 Myxococcus xanthus 5-oxoprolyl-EAA + NH3
-
 ?
2.3.2.5 QFAA
-
 Zymomonas mobilis 5-oxoprolyl-FAA + NH3
-
 ?
2.3.2.5 QFAA
-
 Myxococcus xanthus 5-oxoprolyl-FAA + NH3
-
 ?
2.3.2.5 QGGG
-
 Zymomonas mobilis 5-oxoprolyl-GGG + NH3
-
 ?
2.3.2.5 QGGG
-
 Myxococcus xanthus 5-oxoprolyl-GGG + NH3
-
 ?
2.3.2.5 QRAA
-
 Zymomonas mobilis 5-oxoprolyl-RAA + NH3
-
 ?
2.3.2.5 QRAA
-
 Myxococcus xanthus 5-oxoprolyl-RAA + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.5 ? x * 30000, SDS-PAGE Zymomonas mobilis
2.3.2.5 ? x * 30000, SDS-PAGE Myxococcus xanthus
2.3.2.5 ? x * 29900, MALDI-TOF mass spectrometry Zymomonas mobilis
2.3.2.5 ? x * 31100, MALDI-TOF mass spectrometry Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 glutaminyl cyclase
-
 Zymomonas mobilis
2.3.2.5 glutaminyl cyclase
-
 Myxococcus xanthus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.5 0.00008
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0032
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0042
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0064
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.007
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0074
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.012
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.021
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.054
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.084
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.27
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.45
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 1.1
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 2.7
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 7.1
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 12.8
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 17.9
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 18.9
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 31.7
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 32
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 35.7
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 40
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 40
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 60
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 61.5
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 64.9
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 66
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 102
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 128
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 129
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 170
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 183
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.5 6
-
-
 Zymomonas mobilis
2.3.2.5 8
-
-
 Myxococcus xanthus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.5 0.0008
-
 QAAF in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0013
-
 QARA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0021
-
 QGGG in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0026
-
 QRAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0031
-
 QAFA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0049
-
 L-Gln-L-Glu in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.0067
-
 QEAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.013
-
 L-Gln-L-Gln in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.02
-
 QAEA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.034
-
 QAAE in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 0.24
-
 QFAA in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 1.46
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 4.8
-
 L-Gln-L-Gln in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 5.4
-
 L-Gln-L-Glu in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 6.64
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 8.5
-
 L-Gln-Gly in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 9
-
 QGGG in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 13
-
 L-Gln-amide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 15.7
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 18
-
 QEAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 21.2
-
 L-Gln-amide in 0.1 M MES, pH 6.0, temperature not specified in the publication Zymomonas mobilis
2.3.2.5 31
-
 QFAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 35
-
 L-Gln-2-naphthylamide in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 36
-
 QAAE in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 41
-
 QAFA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 55
-
 QAEA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 63.6
-
 L-Gln-tert-butyl ester in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 76
-
 QRAA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 142
-
 L-Gln-7-amido-4-methylcoumarin in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 219
-
 QAAF in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 412
-
 QAAR in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus
2.3.2.5 1215
-
 QARA in 0.1 M MES, pH 7.5, temperature not specified in the publication Myxococcus xanthus