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Literature summary extracted from

  • May, A.; Berger, S.; Hertel, T.; Koeck, M.
    The Arabidopsis thaliana phosphate starvation responsive gene AtPPsPase1 encodes a novel type of inorganic pyrophosphatase (2011), Biochim. Biophys. Acta, 1810, 178-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 At1g73010, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain Bl21 (DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 D19A site-directed mutagenesis Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.0388
-
 diphosphate pH 8.0, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytosol soluble cytoplasmic enzyme Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ activates to 50% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
3.6.1.1 Fe2+ activates to 88% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
3.6.1.1 Mg2+ dependent on, 10 mM Arabidopsis thaliana
3.6.1.1 Mn2+ activates to 39% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
3.6.1.1 Ni2+ activates to 83% of the activity with Mg2+ at 2 mM Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 33494
-
 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana
3.6.1.1 33500
-
 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana
3.6.1.1 120000
-
 recombinant wild-type enzyme, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Arabidopsis thaliana
-
 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Arabidopsis thaliana Col-0
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Arabidopsis thaliana Q67YC0
-
-
3.6.1.1 Arabidopsis thaliana Col-0 Q67YC0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.1 phosphoprotein the enzyme shares the catalytic mechanism of the HAD superfamily including a phosphorylated enzyme intermediate Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.1 diphosphate + H2O = 2 phosphate the catalytic mechanism of the HAD superfamily including a phosphorylated enzyme intermediate, overview Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 0.00705
-
 purified recombinant wild-type enzyme, pH 8.0, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 2-phosphorylethanolamine + H2O low activity Arabidopsis thaliana ethanolamine + phosphate
-
 ?
3.6.1.1 4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
3.6.1.1 4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana Col-0 4-nitrophenol + phosphate
-
 ?
3.6.1.1 D-glucose-6-phosphate + H2O low activity Arabidopsis thaliana D-glucose + phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Arabidopsis thaliana 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Arabidopsis thaliana Col-0 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana Col-0 2 phosphate
-
 ?
3.6.1.1 additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana ?
-
 ?
3.6.1.1 additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana Col-0 ?
-
 ?
3.6.1.1 thiamine diphosphate + H2O low activity Arabidopsis thaliana ?
-
 ?
3.6.1.1 thiamine diphosphate + H2O low activity Arabidopsis thaliana Col-0 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 tetramer 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 AtPPsPase1
-
 Arabidopsis thaliana
3.6.1.1 inorganic pyrophosphatase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 8
-
 assay at Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.1 Arabidopsis thaliana sequence calculation
-
 5.2

Expression

EC Number Organism Comment Expression
3.6.1.1 Arabidopsis thaliana the enzyme is induced by phosphate starvation up

General Information

EC Number General Information Comment Organism
3.6.1.1 evolution AtPPsPase1 belongs to the haloacid dehalogenase, HAD, superfamily Arabidopsis thaliana
3.6.1.1 additional information modelling of the active site Arabidopsis thaliana
3.6.1.1 physiological function tight control of AtPPsPase1 gene expression underlines its important role in the phosphate starvation response, cleavage of diphosphate is an immediate metabolic adaptation reaction Arabidopsis thaliana