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Literature summary extracted from

  • Chang, H.P.; Liang, W.C.; Lyu, R.C.; Chi, M.C.; Wang, T.F.; Su, K.L.; Hung, H.C.; Lin, L.L.
    Effects of C-terminal truncation on autocatalytic processing of Bacillus licheniformis gamma-glutamyl transpeptidase (2010), Biochemistry, 75, 919-929.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expressed in Escherichia coli M15 cells Bacillus licheniformis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 additional information deletion of up to nine amino acid residues from the C-terminal end of the enzyme definitely affects its autocatalytic activity Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.08
-
 5-L-glutamyl-4-nitroanilide in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 65230
-
 analytical ultracentrifugation Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus licheniformis Q65KZ6
-
-
2.3.2.2 Bacillus licheniformis ATCC 14580 Q65KZ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 Ni2+-NTA resin column chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-2-naphthylamide + glycylglycine
-
 Bacillus licheniformis 5-L-glutamyl-glycylglycine + 2-naphthylamine
-
 ?
2.3.2.2 5-L-glutamyl-2-naphthylamide + glycylglycine
-
 Bacillus licheniformis ATCC 14580 5-L-glutamyl-glycylglycine + 2-naphthylamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus licheniformis 5-L-glutamyl-glycylglycine + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus licheniformis ATCC 14580 5-L-glutamyl-glycylglycine + 4-nitroaniline
-
 ?
2.3.2.2 additional information the enzyme must undergo autocatalytic processing before it has enzymatic activity Bacillus licheniformis ?
-
 ?
2.3.2.2 additional information the enzyme must undergo autocatalytic processing before it has enzymatic activity Bacillus licheniformis ATCC 14580 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 heterodimer
-
 Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyl transpeptidase
-
 Bacillus licheniformis
2.3.2.2 GGT
-
 Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 97
-
 5-L-glutamyl-4-nitroanilide in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.2 1245
-
 5-L-glutamyl-4-nitroanilide in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40°C Bacillus licheniformis