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Literature summary extracted from

  • Kincaid, V.A.; Sullivan, E.D.; Klein, R.D.; Noel, J.W.; Rowlett, R.S.; Snider, M.J.
    Structure and catalytic mechanism of nicotinate (vitamin B3) degradative enzyme maleamate amidohydrolase from Bordetella bronchiseptica RB50 (2012), Biochemistry, 51, 545-554.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.107 expression in Escherichia coli Bordetella bronchiseptica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.107 to 2.4 A resolution. Enzyme belongs to the cysteine hydrolase superfamily. The structure shows a conserved catalytic triad, residues Asp29, Lys117, and Cys150, observed in the proximity of a conserved non-proline cis-peptide bond within a small cavity that is likely the active site Bordetella bronchiseptica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.107 0.128
-
 Maleamate pH 7.5, 25°C Bordetella bronchiseptica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.107 additional information NicF structure and activity do not depend on Zn2+ Bordetella bronchiseptica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.107 44100
-
 4 * 44100, calculated Bordetella bronchiseptica
3.5.1.107 86000
-
 gel fitlration Bordetella bronchiseptica

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.107 Bordetella bronchiseptica A0A0H3LKK8
-
-
3.5.1.107 Bordetella bronchiseptica RB50 A0A0H3LKK8
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.107 maleamate + H2O = maleate + NH3 hydrolysis of maleamate is proposed to proceed by a nucleophilic addition?elimination sequence involving the thiolate side chain of Cys150 Bordetella bronchiseptica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.107 maleamate + H2O
-
 Bordetella bronchiseptica maleate + NH3
-
 ?
3.5.1.107 maleamate + H2O
-
 Bordetella bronchiseptica RB50 maleate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.107 tetramer 4 * 44100, calculated Bordetella bronchiseptica

Synonyms

EC Number Synonyms Comment Organism
3.5.1.107 Bb1774
-
 Bordetella bronchiseptica
3.5.1.107 NicF
-
 Bordetella bronchiseptica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.107 11.7
-
 Maleamate pH 7.5, 25°C Bordetella bronchiseptica