BRENDA - Enzyme Database show

Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae

Smith, B.C.; Anderson, M.A.; Hoadley, K.A.; Keck, J.L.; Cleland, W.W.; Denu, J.M.; Biochemistry 51, 243-256 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.1.19
in complex with nicotinaldehyde, hanging drop vapor diffusion method, using 1.6 M NaOAc, 10% (w/v) ethylene glycol, and 0.1 M HEPES (pH 7.4)
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C167A
the mutant shows most strongly decreased kcat value compared to the wild type enzyme (below the assay detection limit)
Saccharomyces cerevisiae
3.5.1.19
D51A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D51N
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8A
the mutant shows 770fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8E
the mutant shows 100fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8N
the mutant shows 1000fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
H53A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
H94A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
K122A
the mutant shows 16fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
K122R
the mutant shows 770fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-acetylpyridine
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
benzaldehyde
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
additional information
not inhibited by 10 mM EDTA
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
most potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
nicotinic acid
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
pyrazinoic acid
competitive inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0018
-
nicotinamide
Km below 0.0018 mM, mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0024
-
nicotinamide
Km below 0.0024 mM, mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0027
-
nicotinamide
Km below 0.0027 mM, mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0065
-
nicotinamide
Km below 0.0065 mM, mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0096
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.025
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.061
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.157
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
contains tightly bound zinc. Addition of 2 mM ZnCl2 fails to increase the activity of the wild type enzyme
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
best substrate
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Saccharomyces cerevisiae
P53184
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
Ni-NTA column chromatography and Sephacryl S-300 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
1-methylnicotinamide + H2O
-
718924
Saccharomyces cerevisiae
1-methylnicotinate + NH3
-
-
-
?
3.5.1.19
5-methylnicotinamide + H2O
-
718924
Saccharomyces cerevisiae
5-methylnicotinate + NH3
-
-
-
?
3.5.1.19
benzamide + H2O
-
718924
Saccharomyces cerevisiae
benzoate + NH3
-
-
-
?
3.5.1.19
additional information
no activity with nicotinamide mononucleotide and NAD+
718924
Saccharomyces cerevisiae
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
best substrate
718924
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718924
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
thionicotinamide + H2O
-
718924
Saccharomyces cerevisiae
pyridine-3-carbothioic O-acid
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0005
-
nicotinamide
kcat below 0.0005 s-1, mutant enzyme C167A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0006
-
nicotinamide
mutant enzyme D8E, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0009
-
nicotinamide
mutant enzyme D8A, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme D8N, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme K122R, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.007
-
nicotinamide
mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0088
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.014
-
nicotinamide
mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.02
-
nicotinamide
mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.044
-
nicotinamide
mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.064
-
nicotinamide
mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.69
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
1.75
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
2.56
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7
8
-
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.19
0.00094
-
nicotinaldehyde
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0206
-
benzaldehyde
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.12
-
nicotinic acid
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.316
-
3-acetylpyridine
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
6.7
-
pyrazinoic acid
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.1.19
in complex with nicotinaldehyde, hanging drop vapor diffusion method, using 1.6 M NaOAc, 10% (w/v) ethylene glycol, and 0.1 M HEPES (pH 7.4)
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C167A
the mutant shows most strongly decreased kcat value compared to the wild type enzyme (below the assay detection limit)
Saccharomyces cerevisiae
3.5.1.19
D51A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D51N
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8A
the mutant shows 770fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8E
the mutant shows 100fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
D8N
the mutant shows 1000fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
H53A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
H94A
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
K122A
the mutant shows 16fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
3.5.1.19
K122R
the mutant shows 770fold decreased kcat value compared to the wild type enzyme
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-acetylpyridine
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
benzaldehyde
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
additional information
not inhibited by 10 mM EDTA
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
most potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
nicotinic acid
competitive inhibition
Saccharomyces cerevisiae
3.5.1.19
pyrazinoic acid
competitive inhibition
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.19
0.00094
-
nicotinaldehyde
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0206
-
benzaldehyde
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.12
-
nicotinic acid
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.316
-
3-acetylpyridine
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
6.7
-
pyrazinoic acid
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0018
-
nicotinamide
Km below 0.0018 mM, mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0024
-
nicotinamide
Km below 0.0024 mM, mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0027
-
nicotinamide
Km below 0.0027 mM, mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0065
-
nicotinamide
Km below 0.0065 mM, mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0096
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.025
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.061
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.157
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
contains tightly bound zinc. Addition of 2 mM ZnCl2 fails to increase the activity of the wild type enzyme
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
best substrate
nicotinate + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
Ni-NTA column chromatography and Sephacryl S-300 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
1-methylnicotinamide + H2O
-
718924
Saccharomyces cerevisiae
1-methylnicotinate + NH3
-
-
-
?
3.5.1.19
5-methylnicotinamide + H2O
-
718924
Saccharomyces cerevisiae
5-methylnicotinate + NH3
-
-
-
?
3.5.1.19
benzamide + H2O
-
718924
Saccharomyces cerevisiae
benzoate + NH3
-
-
-
?
3.5.1.19
additional information
no activity with nicotinamide mononucleotide and NAD+
718924
Saccharomyces cerevisiae
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
best substrate
718924
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718924
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
thionicotinamide + H2O
-
718924
Saccharomyces cerevisiae
pyridine-3-carbothioic O-acid
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0005
-
nicotinamide
kcat below 0.0005 s-1, mutant enzyme C167A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0006
-
nicotinamide
mutant enzyme D8E, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0009
-
nicotinamide
mutant enzyme D8A, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme D8N, in 50 mM sodium phosphate (pH 7.5), at 25°C; mutant enzyme K122R, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.007
-
nicotinamide
mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0088
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.014
-
nicotinamide
mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.02
-
nicotinamide
mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.044
-
nicotinamide
mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.064
-
nicotinamide
mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.69
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
1.75
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
2.56
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7
8
-
Saccharomyces cerevisiae
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0086
-
1-methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0096
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.025
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.05
-
Thionicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.061
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.157
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.35
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
2.2
-
nicotinamide
mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
6.7
-
nicotinamide
kcat/Km above 6.7 (1/sec*mM), mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
7.4
-
nicotinamide
kcat/Km above 7.4 (1/sec*mM), mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
8.3
-
nicotinamide
kcat/Km above 8.3 (1/sec*mM), mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
16
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
29
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
35
-
nicotinamide
kcat/Km above 35 (1/sec*mM), mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
72
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0086
-
1-methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0096
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.025
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.05
-
Thionicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.061
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.157
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.35
-
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
2.2
-
nicotinamide
mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
6.7
-
nicotinamide
kcat/Km above 6.7 (1/sec*mM), mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
7.4
-
nicotinamide
kcat/Km above 7.4 (1/sec*mM), mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
8.3
-
nicotinamide
kcat/Km above 8.3 (1/sec*mM), mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
16
-
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
29
-
5-Methylnicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
35
-
nicotinamide
kcat/Km above 35 (1/sec*mM), mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae
3.5.1.19
72
-
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
Saccharomyces cerevisiae