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Literature summary extracted from

  • Amari, M.; Arango, L.F.; Gabriel, V.; Robert, H.; Morel, S.; Moulis, C.; Gabriel, B.; Remaud-Simeon, M.; Fontagne-Faucher, C.
    Characterization of a novel dextransucrase from Weissella confusa isolated from sourdough (2013), Appl. Microbiol. Biotechnol., 97, 5413-5422.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.5 food industry the dextransucrase is responsible for production of dextran with predominant alpha-(1->6) linkages that might find applications as food hydrocolloids Weissella confusa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 DNA and amino acid sequence determination and analysis Weissella cibaria
2.4.1.5 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged DSRC39-2 in Escherichia coli strain BL21(DE3) Weissella confusa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information rDSRC39-2 shows Michaelis-Menten-type kinetics Weissella confusa
2.4.1.5 0.0086
-
 sucrose pH 5.4, 30°C, recombinant enzyme Weissella confusa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.5 soluble
-
 Weissella cibaria
-
-
2.4.1.5 soluble
-
 Weissella confusa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa LBAE C39-2
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria LBAE K39
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella cibaria
-
 isolated from wheat sourdough, single copy gene
-
2.4.1.5 Weissella cibaria LBAE K39
-
 isolated from wheat sourdough, single copy gene
-
2.4.1.5 Weissella confusa A0A5E8F621 isolated from wheat sourdough, single copy gene
-
2.4.1.5 Weissella confusa LBAE C39-2 A0A5E8F621 isolated from wheat sourdough, single copy gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant His-tagged DSRC39-2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatogaphy Weissella confusa

Storage Stability

EC Number Storage Stability Organism
2.4.1.5 4°C, purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, about 10% activity loss after 6 days, and about 35% activity loss after 20 days Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella cibaria D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella confusa LBAE C39-2 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella cibaria LBAE K39 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DSR
-
 Weissella cibaria
2.4.1.5 DSR
-
 Weissella confusa
2.4.1.5 DSRC39-2
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35 40
-
 Weissella confusa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 20 30 purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, 30 min, stable, rapid loss of activity above 30°C Weissella confusa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.5 60
-
 sucrose pH 5.4, 30°C, recombinant enzyme Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
-
 Weissella confusa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.5 Weissella confusa sequence calculation
-
 4.6

General Information

EC Number General Information Comment Organism
2.4.1.5 evolution dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 Weissella cibaria
2.4.1.5 evolution dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 Weissella confusa
2.4.1.5 additional information the amino acids involved in catalysis, E592, D554, D665 are conserved in the catalytic core Weissella confusa
2.4.1.5 physiological function the dextransucrase is responsible for production of dextran with predominant alpha-(1->) linkages Weissella confusa