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Literature summary extracted from

  • Cecchini, D.A.; Pavesi, R.; Sanna, S.; Daly, S.; Xaiz, R.; Pregnolato, M.; Terreni, M.
    Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase (2012), Appl. Microbiol. Biotechnol., 95, 1491-1500.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.11 alphaF146Y bad catalyst for synthesis Escherichia coli
3.5.1.11 betaF24A reduced hydrolytic activity towards amides of phenylglycine Escherichia coli
3.5.1.11 betaF24A/alphaF146Y very low amidase activity Escherichia coli
3.5.1.11 betaF24T used as negative control because of the negative effect on synthetic and hydrolytic activities Escherichia coli
3.5.1.11 betaF24T/alphaF146Y used as negative control because of the negative effect on synthetic and hydrolytic activities Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli P06875
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 ion exchange chromatography (Q-Sepharose) Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 0.7
-
 substrate penicillin G, betaF24A/alphaF146Y mutant protein, pH 8.0, 37°C Escherichia coli
3.5.1.11 3 8 substrate penicillin G, wild type protein, pH 8.0, 37°C Escherichia coli
3.5.1.11 11
-
 substrate penicillin G, betaF24A mutant protein, pH 8.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 6-nitro-3-(phenylacetamido)benzoic acid + H2O
-
 Escherichia coli ?
-
 ?
3.5.1.11 7-amino-3-propenyl-cephalosporanic acid + 4-hydroxy-D-phenylglycine methyl ester
-
 Escherichia coli cefprozil + ?
-
 ?
3.5.1.11 D-phenylglycine amide + 7-aminodeacetoxycephalosporanic acid
-
 Escherichia coli cephalexin + ?
-
 ?
3.5.1.11 D-phenylglycine amide + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
-
 Escherichia coli cephaclor + ?
-
 ?
3.5.1.11 D-phenylglycine methyl ester + 7-aminodeacetoxycephalosporanic acid
-
 Escherichia coli cephalexin + ?
-
 ?
3.5.1.11 D-phenylglycine methyl ester + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
-
 Escherichia coli cephaclor + ?
-
 ?
3.5.1.11 penicillin G + H2O
-
 Escherichia coli phenyl acetic acid + 6-aminopenicillanate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin acylase
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.11 10
-
 stable: 2 h at 20°C or 4°C, mutant betaF24A and mutant betaF24A/alphaF146Y: more than 80% activity after 2 h at 4°C, less than 10% activity after 2 h at 20°C Escherichia coli