Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yokoyama, K.; Utsumi, H.; Nakamura, T.; Ogaya, D.; Shimba, N.; Suzuki, E.; Taguchi, S.
    Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis (2010), Appl. Microbiol. Biotechnol., 87, 2087-2096.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 additional information the enzyme is secreted as a pro-enzyme which is activated by the endoprotease TAMEP Streptomyces mobaraensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Corynebacterum glutamicum strain ATTC13869 Streptomyces mobaraensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 A10S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 D14N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 D3F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 D3L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 D3N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 E28D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 E58D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 H289F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 H289Y the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 M16T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 M16T/G283S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N320D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N32D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N32D/E264D/N320T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 P12S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Q74A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Q74L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Q74N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 R238F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 R238L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 R26F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 R26L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 R5K the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S199A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S284T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S299L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S303A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S303F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 S303T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 T77A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 T77F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 T77L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 T77S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V30D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V30I the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V30T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V65I the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V6T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 W59F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y34F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y34F/D268N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y42H the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y75A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y75F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y75H the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ dependent on Streptomyces mobaraensis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces mobaraensis
-
-
-
2.3.2.13 Streptomyces mobaraensis s-8112
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Resource S column chromatography and Sephadex G25 gel filtration Streptomyces mobaraensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 27.2
-
 wild type enzyme, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 27.4
-
 mutant enzyme N320D, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 28.5
-
 mutant enzyme Y42H, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 28.6
-
 mutant enzyme Y34F/D268N, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 28.7
-
 mutant enzyme N32D/E264D/N320T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 28.9
-
 mutant enzyme N32D, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 29.1
-
 mutant enzyme V30D, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 29.7
-
 mutant enzyme A10S, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 29.8
-
 mutant enzyme M16T/G283S, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.1
-
 mutant enzyme Q74L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.2
-
 mutant enzyme V6T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.3
-
 mutant enzyme R238F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.4
-
 mutant enzyme R26L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.5
-
 mutant enzyme D14N, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.5
-
 mutant enzyme V30T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.6
-
 mutant enzyme S303A, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.6
-
 mutant enzyme S303F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.7
-
 mutant enzyme R238L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 30.9
-
 mutant enzyme S284T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 31.4
-
 mutant enzyme T77L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 31.6
-
 mutant enzyme Y75H, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.1
-
 mutant enzyme S299L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.3
-
 mutant enzyme E28D, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.3
-
 mutant enzyme P12S, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.4
-
 mutant enzyme D3L, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.4
-
 mutant enzyme S303T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 32.7
-
 mutant enzyme E58D, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 33
-
 mutant enzyme H289Y, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 33.4
-
 mutant enzyme R5K, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 33.5
-
 mutant enzyme D3N, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 33.6
-
 mutant enzyme T77A, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 33.8
-
 mutant enzyme V30I, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 34.1
-
 mutant enzyme T77F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 34.3
-
 mutant enzyme Y34F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 34.4
-
 mutant enzyme Q74N, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 34.9
-
 mutant enzyme H289F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 35.5
-
 mutant enzyme R26F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 35.5
-
 mutant enzyme Y75A, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 35.8
-
 mutant enzyme D3F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 35.9
-
 mutant enzyme W59F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 36
-
 mutant enzyme V65I, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 36.6
-
 mutant enzyme T77S, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 37.4
-
 mutant enzyme Q74A, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 41.7
-
 mutant enzyme Y75F, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 42.5
-
 mutant enzyme M16T, at pH 6.0 and 37°C Streptomyces mobaraensis
2.3.2.13 42.9
-
 mutant enzyme S199A , at pH 6.0 and 37°C Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N-carbobenzoxy-L-glutaminylglycine + NH2OH
-
 Streptomyces mobaraensis hydroxamic acid + ?
-
 ?
2.3.2.13 N-carbobenzoxy-L-glutaminylglycine + NH2OH
-
 Streptomyces mobaraensis s-8112 hydroxamic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 MTG
-
 Streptomyces mobaraensis
2.3.2.13 protein-glutamine gamma-glutamyltransferase
-
 Streptomyces mobaraensis
2.3.2.13 TGase
-
 Streptomyces mobaraensis