Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mao, X.; Wang, S.; Kan, F.; Wei, D.; Li, F.
    A novel dextran dextrinase from Gluconobacter oxydans DSM-2003: purification and properties (2012), Appl. Biochem. Biotechnol., 168, 1256-1264.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.2 Co2+
-
 Gluconobacter oxydans
2.4.1.2 Cu2+ strong inhibition Gluconobacter oxydans
2.4.1.2 Fe2+ strong inhibition Gluconobacter oxydans
2.4.1.2 Ni2+
-
 Gluconobacter oxydans
2.4.1.2 Zn2+ strong inhibition Gluconobacter oxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.2 Ba2+ slight activation Gluconobacter oxydans
2.4.1.2 Ca2+ slight activation Gluconobacter oxydans
2.4.1.2 Mg2+ slight activation Gluconobacter oxydans
2.4.1.2 Mn2+ slight activation Gluconobacter oxydans
2.4.1.2 additional information no effect by Fe3+ Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.2 62000
-
 gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.2 additional information Gluconobacter oxydans DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation ?
-
 ?
2.4.1.2 additional information Gluconobacter oxydans DSM 2003 DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation ?
-
 ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m Gluconobacter oxydans
-
 [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m Gluconobacter oxydans DSM 2003
-
 [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.2 Gluconobacter oxydans
-
-
-
2.4.1.2 Gluconobacter oxydans DSM 2003
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.2 native DDase 187.5fold to homogeneity by anion exchange chromatography and gel filtration Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.2 112.5
-
 purified enzyme, pH 6.5, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.2 4-nitrophenyl-alpha-D-glucopyranoside + H2O artificial substrate Gluconobacter oxydans 4-nitrophenol + alpha-D-glucopyranose
-
 ?
2.4.1.2 4-nitrophenyl-alpha-D-glucopyranoside + H2O artificial substrate Gluconobacter oxydans DSM 2003 4-nitrophenol + alpha-D-glucopyranose
-
 ?
2.4.1.2 additional information DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation Gluconobacter oxydans ?
-
 ?
2.4.1.2 additional information DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation Gluconobacter oxydans DSM 2003 ?
-
 ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
-
 Gluconobacter oxydans [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
-
 ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
-
 Gluconobacter oxydans [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
-
 Gluconobacter oxydans DSM 2003 [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
-
 ?
2.4.1.2 [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
-
 Gluconobacter oxydans DSM 2003 [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points ?

Subunits

EC Number Subunits Comment Organism
2.4.1.2 monomer 1 * 62000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.2 DDase
-
 Gluconobacter oxydans
2.4.1.2 dextran dextrinase
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.2 30
-
-
 Gluconobacter oxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.2 11 35 sharp decrease at temperatures higher than 35 °C Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.2 30
-
 stable below 30°C for 3 h Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.2 6.3
-
-
 Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.2 5 9 stable within this range for 3 h Gluconobacter oxydans