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Literature summary extracted from

  • Goncalves, V.; Brannigan, J.A.; Thinon, E.; Olaleye, T.O.; Serwa, R.; Lanzarone, S.; Wilkinson, A.J.; Tate, E.W.; Leatherbarrow, R.J.
    A fluorescence-based assay for N-myristoyltransferase activity (2012), Anal. Biochem., 421, 342-344.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
-
 Homo sapiens
2.3.1.97 N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.00276
-
 peptide Hs pp60src(2-9) pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens
2.3.1.97 0.00277
-
 peptide Hs pp60src(2-9) pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens
2.3.1.97 0.00724
-
 myristoyl-CoA pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens
2.3.1.97 0.00824
-
 myristoyl-CoA pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 tetradecanoyl-CoA + glycylpeptide Homo sapiens posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins CoA + N-tetradecanoylglycylpeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Homo sapiens
-
 isozymes NMT1 and NMT2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 additional information development of a robust sensitive fluorescence-based assay method to study the enzymatic activity of NMT1 and NMT2 based on detection of CoA by 7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin, overview Homo sapiens ?
-
 ?
2.3.1.97 myristoyl-CoA + peptide Hs pp60src(2-9)
-
 Homo sapiens CoA + N-myristoyl-peptideHs pp60src(2-9)
-
 ?
2.3.1.97 tetradecanoyl-CoA + glycylpeptide posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens CoA + N-tetradecanoylglycylpeptide
-
 ?
2.3.1.97 tetradecanoyl-CoA + glycylpeptide transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens CoA + N-tetradecanoylglycylpeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 myristoyl-CoA:protein N-myristoyltransferase
-
 Homo sapiens
2.3.1.97 myristoyl-coenzyme A:protein N-myristoyltransferase
-
 Homo sapiens
2.3.1.97 N-myristoyltransferase
-
 Homo sapiens
2.3.1.97 NMT
-
 Homo sapiens
2.3.1.97 NMT1
-
 Homo sapiens
2.3.1.97 NMT2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.97 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.97 7.9 8 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.97 0.0000137
-
 pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
2.3.1.97 0.0000144
-
 pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
2.3.1.97 0.000349
-
 pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide
2.3.1.97 0.000513
-
 pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide

General Information

EC Number General Information Comment Organism
2.3.1.97 physiological function myristoyl-CoA:protein N-myristoyltransferase is a ubiquitous enzyme in eukaryotes that catalyzes the co- and posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens