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Literature summary extracted from

  • Shabalin, I.G.; Porebski, P.J.; Cooper, D.R.; Grabowski, M.; Onopriyenko, O.; Grimshaw, S.; Savchenko, A.; Chruszcz, M.; Minor, W.
    Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution (2012), Acta Crystallogr. Sect. F, 68, 1018-1024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Campylobacter jejuni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.3 unliganded aOTC, hanging drop vapor diffusion method, mixing of 500 nl 10 mg/ml protein solution with 500 nl well solution containing 25% PEG 3350, 0.2 M NaCl, 0.1 M HEPES, pH 8.0, room temperature, 1 week, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement and modeling Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Campylobacter jejuni
-
 L-citrulline + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine Campylobacter jejuni NCTC 11168
-
 L-citrulline + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Campylobacter jejuni Q9PNU6 subsp. jejuni, gene argF
-
2.1.3.3 Campylobacter jejuni NCTC 11168 Q9PNU6 subsp. jejuni, gene argF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Campylobacter jejuni L-citrulline + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine the mechanism of the aOTC-catalyzed reaction involves nucleophilic attack of the electron pair of the epsilon-amino group of L-ornithine on the carbonyl group of carbamoyl phosphate Campylobacter jejuni L-citrulline + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Campylobacter jejuni NCTC 11168 L-citrulline + phosphate
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine the mechanism of the aOTC-catalyzed reaction involves nucleophilic attack of the electron pair of the epsilon-amino group of L-ornithine on the carbonyl group of carbamoyl phosphate Campylobacter jejuni NCTC 11168 L-citrulline + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer forms a head-to-head pseudohexamer in the asymmetric unit, each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. Conformation of the B2-H3 loop, residues 68-78, is involved in binding CP in an adjacent subunit of the trimer, overview Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 anabolic ornithine transcarbamoylase
-
 Campylobacter jejuni
2.1.3.3 AOTC
-
 Campylobacter jejuni
2.1.3.3 ornithine carbamoyltransferase
-
 Campylobacter jejuni
2.1.3.3 OTC
-
 Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.1.3.3 evolution aOTC is a widespread enzyme that is found in a large variety of organisms from bacteria to mammals Campylobacter jejuni
2.1.3.3 metabolism anabolic ornithine transcarbamoylase is involved in the urea cycle and L-arginine biosynthesis Campylobacter jejuni
2.1.3.3 additional information the conserved active site of Campylobacter jejuni aOTC consists of residues from both carbamoyl phosphate binding and L-ornithine-binding domains of the subunit and the B2-H3 loop, residues 68-78, from an adjacent subunit of the trimer, active site structure, overview Campylobacter jejuni
2.1.3.3 physiological function OTC catalyzes the reversible transfer of the carbamoyl group from carbamoyl phosphate to the Nepsilon atom of L-ornithine to produce L-citrulline. There are two types of enzyme: anabolic, aOTC, and catabolic, cOTC. Anabolic OTCs catalyze the forward reaction and participate in the urea cycle and L-arginine biosynthesis Campylobacter jejuni