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Literature summary extracted from

  • Liu, W.; Shen, W.; Zhao, X.; Cao, H.; Cui, Z.
    Expression, purification, crystallization and preliminary X-ray analysis of para-nitrophenol 4-monooxygenase from Pseudomonas putida DLL-E4 (2009), Acta Crystallogr. Sect. F, 65, 1004-1006.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.166 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida
1.14.13.167 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.166 sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol Pseudomonas putida
1.14.13.167 sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.166 46600
-
 x * 46600, calculated from amino acid sequence Pseudomonas putida
1.14.13.167 46600
-
 x * 46600, calculated from amino acid sequence Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.166 Pseudomonas putida C6FI48
-
-
1.14.13.166 Pseudomonas putida DLL-E4 C6FI48
-
-
1.14.13.167 Pseudomonas putida C6FI48
-
-
1.14.13.167 Pseudomonas putida DLL-E4 C6FI48
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.166 Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration Pseudomonas putida
1.14.13.167 Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.166 4-nitrocatechol + NADH + H+ + O2
-
 Pseudomonas putida 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
1.14.13.166 4-nitrocatechol + NADH + H+ + O2
-
 Pseudomonas putida DLL-E4 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
1.14.13.167 4-nitrophenol + NADH + H+ + O2
-
 Pseudomonas putida 1,4-benzoquinone + nitrite + NAD+ + H2O
-
 ?
1.14.13.167 4-nitrophenol + NADH + H+ + O2
-
 Pseudomonas putida DLL-E4 1,4-benzoquinone + nitrite + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.166 ? x * 46600, calculated from amino acid sequence Pseudomonas putida
1.14.13.167 ? x * 46600, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.166 para-nitrophenol 4-monooxygenase
-
 Pseudomonas putida
1.14.13.166 PNP 4-monooxygenase
-
 Pseudomonas putida
1.14.13.166 pnpA
-
 Pseudomonas putida
1.14.13.167 para-nitrophenol 4-monooxygenase
-
 Pseudomonas putida
1.14.13.167 PNP 4-monooxygenase
-
 Pseudomonas putida
1.14.13.167 pnpA
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.166 FAD
-
 Pseudomonas putida
1.14.13.166 NADH
-
 Pseudomonas putida
1.14.13.167 FAD
-
 Pseudomonas putida
1.14.13.167 NADH
-
 Pseudomonas putida