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Literature summary extracted from

  • Costa, J.; Fonseca, K.; Neves Mamede, C.; Beletti, M.; Santos-Filho, N.; Soares, A.; Arantes, E.; Hirayama, S.; Selistre-de-Araujo, H.; Fonseca, F.; Henrique-Silva, F.; Penha-Silva, N.; Oliveira, F.
    Bhalternin: Functional and structural characterization of a new thrombin-like enzyme from Bothrops alternatus snake venom (2010), Toxicon, 55, 1365-1377.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.74 pharmacology Bhaltenin may be of interest as a therapeutic agent in the treatment and prevention of thrombotic disorders Bothrops alternatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.74 DNA and amino acid sequence determination and analysis Bothrops alternatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.74 benzamidine
-
 Bothrops alternatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.74 extracellular
-
 Bothrops alternatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.74 27000
-
 1 * 27000, SDS-PAGE Bothrops alternatus
3.4.21.74 31500
-
 native PAGE Bothrops alternatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.74 Fibrinogen + H2O Bothrops alternatus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.74 Bothrops alternatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.74 native enzyme from venom by anion exchange chromatography, gel filtration, and benzamidine affinity chromatography Bothrops alternatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.74 venom
-
 Bothrops alternatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.74 Albumin + H2O bovine substrate Bothrops alternatus ?
-
 ?
3.4.21.74 azocasein + H2O
-
 Bothrops alternatus ?
-
 ?
3.4.21.74 Fibrinogen + H2O
-
 Bothrops alternatus ?
-
 ?
3.4.21.74 Fibrinogen + H2O bovine substrate, the enzyme cleaves preferentially the Aalpha-chain, apparently not degrading the Bbeta and gamma-chains Bothrops alternatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.74 monomer 1 * 27000, SDS-PAGE Bothrops alternatus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.74 Bhalternin
-
 Bothrops alternatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.74 30 40
-
 Bothrops alternatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.74 7 8
-
 Bothrops alternatus

General Information

EC Number General Information Comment Organism
3.4.21.74 additional information the enzyme is a thrombin-like serine protease Bothrops alternatus
3.4.21.74 physiological function the enzyme causes morphological alterations in heart, liver, lung and muscle of mice and causes blood clotting in vitro and defibrinogenation when intraperitoneally administered to mice, suggesting it to be a thrombin-like enzyme Bothrops alternatus