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Literature summary extracted from

  • Llerena-Suster, C.; Jose, C.; Collins, S.; Briand, L.; Morcelle, S.
    Investigation of the structure and proteolytic activity of papain inaqueous miscible organic media (2012), Process Biochem., 47, 47-56.
No PubMed abstract available

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.2 acetonitrile papain retains almost all its catalytic activity after 24 h of incubation in the presence of 99% (v/v) acetonitrile with a more compact conformation Carica papaya
3.4.22.2 dimethyl formamide papain shows a complete inactivation after 24 h when exposed to those media containing 90% (v/v) dimethyl formamide because of an irreversible conformational change Carica papaya
3.4.22.2 Methanol papain shows 80% loss of activity after 24 h incubation in 90% (v/v) methanol although no global conformational change and minor secondary structure rearrangements are detected Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.2
-
 Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 Bovine serum albumin + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 casein + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 DL-4-hydroxyphenylglycine methyl ester + H2O asymmetric hydrolysis Carica papaya ?
-
 ?
3.4.22.2 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Carica papaya L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.2 additional information papain is also able to synthesize L-aminoacylantipyrine amides, Z-Gly-Phe-NH2 and Boc-Gly-Phe-OMe, and performs hydrogenation of methyl 2-acetamidoacrylate Carica papaya ?
-
 ?
3.4.22.2 Nalpha-benzoyl-L-Arg ethyl ester
-
 Carica papaya ?
-
 ?
3.4.22.2 ovalbumin + H2O
-
 Carica papaya ?
-
 ?