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Literature summary extracted from

  • Perez-Sanchez, G.; Leal-Guadarrama, L.; Trelles, I.; Perez, N.; Medina-Rivero, E.
    High-level production of a recombinant Vibrio proteolyticus leucine aminopeptidase and its use for N-terminal methionine excision from interferon alpha-2b (2011), Process Biochem., 46, 1825-1830.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.10 His-tagged protein expressed in Escherichia coli MM294 Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.02726
-
 recombinant human methionine-interferon alpha-2b pH 6.0, 37°C Vibrio proteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 32000
-
 x * 32000, SDS-PAGE Vibrio proteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Vibrio proteolyticus Q01693
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 from inclusion bodies, immobilized metal ion affinity chromatography (Ni2+), purity of 95% Vibrio proteolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 16
-
 substrate L-leucine-4-nitroanilidepH 8.0, 37°C Vibrio proteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-leucine-4-nitroanilide + H2O
-
 Vibrio proteolyticus L-leucine + 4-nitroaniline
-
 ?
3.4.11.10 recombinant human methionine-interferon alpha-2b + H2O
-
 Vibrio proteolyticus L-methionine + recombinant human interferon alpha-2b
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 ? x * 32000, SDS-PAGE Vibrio proteolyticus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 leucine aminopeptidase
-
 Vibrio proteolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 37
-
-
 Vibrio proteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 0.000853
-
 recombinant human methionine-interferon alpha-2b pH 6.0, 37°C Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 6
-
-
 Vibrio proteolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.10 4 9
-
 Vibrio proteolyticus