Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yu, F.; Okamto, S.; Nakasone, K.; Adachi, K.; Matsuda, S.; Harada, H.; Misawa, N.; Utsumi, R.
    Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith) (2008), Planta, 227, 1291-1299.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.104 expressed in Escherichia coli BL21-CodonPlus (DE3) cells Zingiber zerumbet

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.104 64500
-
 x * 64500, calculated from amino acid sequence Zingiber zerumbet

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.104 Zingiber zerumbet
-
 shampoo ginger
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.104 Ni-NTA column chromatography, gel filtration Zingiber zerumbet

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.104 leaf
-
 Zingiber zerumbet
-
4.2.3.104 additional information not detected in stem Zingiber zerumbet
-
4.2.3.104 rhizome highest expression in rhizomes Zingiber zerumbet
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate
-
 Zingiber zerumbet alpha-humulene + diphosphate 95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%) ?

Subunits

EC Number Subunits Comment Organism
4.2.3.104 ? x * 64500, calculated from amino acid sequence Zingiber zerumbet

Synonyms

EC Number Synonyms Comment Organism
4.2.3.104 alpha-humulene synthase
-
 Zingiber zerumbet
4.2.3.104 ZSS1
-
 Zingiber zerumbet

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.104 Zingiber zerumbet calculated from amino acid sequence
-
 5.3

Expression

EC Number Organism Comment Expression
4.2.3.104 Zingiber zerumbet the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate up

General Information

EC Number General Information Comment Organism
4.2.3.104 metabolism alpha-humulene synthase is a key enzyme of zerumbone biosynthesis Zingiber zerumbet