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Literature summary extracted from

  • Rosloniec, K.; Wilbrink, M.; Capyk, J.; Mohn, W.; Ostendorf, M.; Van Der Geize, R.; Dijkhuizen, L.; Eltis, L.
    Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1 (2009), Mol. Microbiol., 74, 1031-1043.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.29 expressed in Escherichia coli BL21(DE3) cells Rhodococcus jostii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.29 47200
-
 x * 47200, calculated from amino acid sequence Rhodococcus jostii

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.29 Rhodococcus jostii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.29 Ni2+-NTA column chromatography Rhodococcus jostii

Subunits

EC Number Subunits Comment Organism
1.14.15.29 ? x * 47200, calculated from amino acid sequence Rhodococcus jostii

Synonyms

EC Number Synonyms Comment Organism
1.14.15.29 CYP125
-
 Rhodococcus jostii
1.14.15.29 CYP125A1
-
 Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.29 heme
-
 Rhodococcus jostii
1.14.15.29 NADH
-
 Rhodococcus jostii

General Information

EC Number General Information Comment Organism
1.14.15.29 metabolism CYP125 performs an obligate first step in cholesterol degradation. CYP125 is involved in steroid C26-carboxylic acid formation, catalysing the oxidation of C26 either to the corresponding carboxylic acid or to an intermediate state Rhodococcus jostii
1.14.15.29 physiological function cyp125 is essential for Rhodococcus jostii strain RHA1 to grow on 3-hydroxysterols such as cholesterol, but not on 3-oxo sterol derivatives Rhodococcus jostii