EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.30 | additional information | unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected | Rhodococcus erythropolis |
1.14.15.30 | additional information | unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation | Rhodococcus erythropolis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.30 | Fe2+ | contains non-heme iron | Rhodococcus erythropolis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.30 | Rhodococcus erythropolis | Q8KQH9 | component KshB of 3-ketosteroid 9alpha-hydroxylase | - |
1.14.15.30 | Rhodococcus erythropolis | Q8KQI0 | component KshA of 3-ketosteroid 9alpha-hydroxylase | - |
1.14.15.30 | Rhodococcus erythropolis SQ1 | Q8KQH9 | component KshB of 3-ketosteroid 9alpha-hydroxylase | - |
1.14.15.30 | Rhodococcus erythropolis SQ1 | Q8KQI0 | component KshA of 3-ketosteroid 9alpha-hydroxylase | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.30 | 4-androstene-3,17-dione + O2 + NADH + H+ | - |
Rhodococcus erythropolis | 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+ | - |
? | |
1.14.15.30 | 4-androstene-3,17-dione + O2 + NADH + H+ | - |
Rhodococcus erythropolis SQ1 | 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+ | - |
? | |
1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus erythropolis | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus erythropolis SQ1 | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.30 | 3-ketosteroid 9alpha-hydroxylase | - |
Rhodococcus erythropolis |
1.14.15.30 | KSH | - |
Rhodococcus erythropolis |
1.14.15.30 | KshA | terminal oxygenase component of 3-ketosteroid 9alpha-hydroxylase, kshA encodes both androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione 9alpha-hydroxylase activity | Rhodococcus erythropolis |
1.14.15.30 | KshB | the KshB protein can be assigned to the class IA oxygenase ferredoxin reductases and represents the ferredoxin reductase component of 3-ketosteroid 9alpha-hydroxylase | Rhodococcus erythropolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.30 | [2Fe-2S]-center | - |
Rhodococcus erythropolis |