BRENDA - Enzyme Database

Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1

Van Der Geize, R.; Hessels, G.; Van Gerwen, R.; Van Der Meijden, P.; Dijkhuizen, L.; Mol. Microbiol. 45, 1007-1018 (2002)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.30
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected; unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation
Rhodococcus erythropolis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.30
Fe2+
contains non-heme iron; contains non-heme iron
Rhodococcus erythropolis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.30
Rhodococcus erythropolis
Q8KQH9
component KshB of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30
Rhodococcus erythropolis
Q8KQI0
component KshA of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30
Rhodococcus erythropolis SQ1
Q8KQH9
component KshB of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30
Rhodococcus erythropolis SQ1
Q8KQI0
component KshA of 3-ketosteroid 9alpha-hydroxylase
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.30
[2Fe-2S]-center
;
Rhodococcus erythropolis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.30
[2Fe-2S]-center
-
Rhodococcus erythropolis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.30
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected
Rhodococcus erythropolis
1.14.15.30
additional information
unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation
Rhodococcus erythropolis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.30
Fe2+
contains non-heme iron
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
718125
Rhodococcus erythropolis SQ1
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?