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Literature summary extracted from
- beta-Xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans (2012), Lett. Appl. Microbiol., 54, 79-87.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | biotechnology | the bifunctional beta-glucosidase/xylosidase can be used in simultaneous saccharification of cellulose and xylan into fermenantable glucose and xylose | uncultured rumen bacterium |
| 3.2.1.37 | biotechnology | the bifunctional beta-glucosidase/xylosidase can be used in simultaneous saccharification of cellulose ans xylan into fermenantable glucose and xylose | uncultured rumen bacterium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.164 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, compared to KM-value: 0.03 mM for 4-nitrophenyl-beta-D-xylopyranoside of the beta-xylosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate | uncultured rumen bacterium |  |
| 3.2.1.37 | 0.03 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 50°C, compared to KM-value: 0.164 mM for 4-nitrophenyl-beta-D-glucopyranoside of the beta-glucosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate | uncultured rumen bacterium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 80000 | - |
purified RuBGX1-6His | uncultured rumen bacterium |
| 3.2.1.37 | 80000 | - |
purified RuBGX1-6His | uncultured rumen bacterium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | uncultured rumen bacterium | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | uncultured rumen bacterium | native beta-glucosidase substrate, 26.4% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | 2 D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | uncultured rumen bacterium | D3VYD8 | RuBGX1, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21, as D272 is the catalytic nucleophile, and E167 and E481 are the catalytic acids; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
| 3.2.1.37 | uncultured rumen bacterium | D3VYD8 | RuBGX1, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21, three catalytic amino acids of RuBGX1 are identified , as D272 is the catalytic nucleophile, and E167 and E481 are the catalytic acids; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured rumen bacterium | - |
| 3.2.1.37 | additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured rumen bacterium | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| 3.2.1.21 | 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-galactopyranoside + H2O | 8.3% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-nitrophenol + D-galactose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | 100% relative activity | uncultured rumen bacterium | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | native beta-glucosidase substrate, 26.4% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 2 D-glucose | - |
? | |
| 3.2.1.21 | additional information | no activity using 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, or sucrose as substrates | uncultured rumen bacterium | ? | - |
? | |
| 3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + H2O | 29% of its beta-glucosidase activity against p-nitrophenyl-beta-D-glucopyranoside | uncultured rumen bacterium | 4-nitrophenol + D-xylose | - |
? | |
| 3.2.1.37 | additional information | the hydrolysis of xylooligosaccharide substrates is investigated using an endoxylanase-coupled assay, functions synergistically to promote the degradation of hemicellulosic xylans | uncultured rumen bacterium | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | beta-glucosidase | belongs to glycosyl hydrolase family 3, GH3 | uncultured rumen bacterium |
| 3.2.1.21 | bifunctional beta-glucosidase/xylosidase | - |
uncultured rumen bacterium |
| 3.2.1.21 | RuBGX1 | gene name, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21 | uncultured rumen bacterium |
| 3.2.1.37 | beta-xylosidase | - |
uncultured rumen bacterium |
| 3.2.1.37 | bifunctional beta-glucosidase/xylosidase | belongs to glycosyl hydrolase family 3, GH3 | uncultured rumen bacterium |
| 3.2.1.37 | RuBGX1 | gene name, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21 | uncultured rumen bacterium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
a narrow optimum around 50°C | uncultured rumen bacterium |
| 3.2.1.37 | 45 | - |
assay at | uncultured rumen bacterium |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 43 | 52 | 60% activity at 43°C, and 65% at 52°C, using p-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
loses 38% activity after 10 min | uncultured rumen bacterium |
| 3.2.1.21 | 55 | - |
loses 90% after 3 min, indicating the RuBGX1 is a mesophilic enzyme | uncultured rumen bacterium |
| 3.2.1.21 | 60 | - |
loses 90% after 3 min, indicating the RuBGX1 is a mesophilic enzyme | uncultured rumen bacterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6 | - |
4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium |
| 3.2.1.37 | 6 | - |
assay at | uncultured rumen bacterium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 8 | activity dropped to 22% at pH 8.0 and to near zero at pH 4.0 | uncultured rumen bacterium |
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