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Literature summary extracted from

  • Bai, Y.; Wang, J.; Zhang, Z.; Yang, P.; Shi, P.; Luo, H.; Meng, K.; Huang, H.; Yao, B.
    A new xylanase from thermoacidophilic Alicyclobacillus sp. A4 with broad-range pH activity and pH stability (2010), J. Ind. Microbiol. Biotechnol., 37, 187-194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol activates at 1 mM Alicyclobacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynA4, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Alicyclobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Alicyclobacillus sp.
3.2.1.8 Hg2+
-
 Alicyclobacillus sp.
3.2.1.8 Mn2+
-
 Alicyclobacillus sp.
3.2.1.8 Ni2+
-
 Alicyclobacillus sp.
3.2.1.8 SDS
-
 Alicyclobacillus sp.
3.2.1.8 Zn2+
-
 Alicyclobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ activates at 1 mM Alicyclobacillus sp.
3.2.1.8 Co2+ activates at 1 mM Alicyclobacillus sp.
3.2.1.8 Cr3+ activates at 1 mM Alicyclobacillus sp.
3.2.1.8 Li+ activates at 1 mM Alicyclobacillus sp.
3.2.1.8 Mg2+ activates at 1 mM Alicyclobacillus sp.
3.2.1.8 additional information the enzyme is unaffected by 1-10 mM K+, Na+, and EDTA Alicyclobacillus sp.
3.2.1.8 Pb2+ activates at 1 mM Alicyclobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 42500
-
 x * 42500, about, sequence calculation Alicyclobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Alicyclobacillus sp. D3VWB5 isolated from a hot spring in Baoshan City, Yunnan Province, China, gene xynA4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Alicyclobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birch wood xylan + H2O
-
 Alicyclobacillus sp. ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Alicyclobacillus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 42500, about, sequence calculation Alicyclobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-1,4-beta-xylanase
-
 Alicyclobacillus sp.
3.2.1.8 XynA4
-
 Alicyclobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
-
 Alicyclobacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 65 XynA4 shows over 50% of maximal activity within this range Alicyclobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 purified recombinant XynA4, 1 h, pH 7.0, over 90% activity remaining, half-life is 394 min Alicyclobacillus sp.
3.2.1.8 65
-
 purified recombinant XynA4, half-life is 17 min Alicyclobacillus sp.
3.2.1.8 70
-
 purified recombinant XynA4, half-life is 3 min Alicyclobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 Alicyclobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3.8 9.4 XynA4 shows over 40% of maximal activity within this range Alicyclobacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 2.6 12 purified recombinant XynA4, 1 h, 37°C, over 80% remaining activity Alicyclobacillus sp.