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Literature summary extracted from

  • Ferguson Jr., D.; Krzycki, J.; Grahame, D.
    Specific roles of methylcobamide:coenzyme M methyltransferase isozymes in metabolism of methanol and methylamines in Methanosarcina barkeri (1996), J. Biol. Chem., 271, 5189-5194.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.247 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
-
 ?
2.1.1.247 a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
-
 ?
2.1.1.247 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.247 Methanosarcina barkeri
-
 isozymes MT2-M and MT2-A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.247 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
-
 ?
2.1.1.247 a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
-
 ?
2.1.1.247 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.247 methylcobamide:coenzyme M methyltransferase
-
 Methanosarcina barkeri
2.1.1.247 MT2-A
-
 Methanosarcina barkeri
2.1.1.247 MT2-M
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.247 37
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.247 7 8 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.247 malfunction immunosorptive depletion of MT2 isozymes from cell-free extracts, extracts of methanol-grown cells depleted of MT2-M lose entirely the ability to carry out conversion of methanol to 2-(methylthio)ethanesulfonate, i.e. methyl-CoM. Methanol:CoM methyl transfer activity is completely restored by addition of purified MT2-M, but no activity is recovered by addition of MT2-A. In contrast, the activity of trimethylamine-grown cell extracts to convert monomethylamine and dimethylamine to methyl-CoM is lost almost entirely by immunosorptive removal of MT2-A. Addition of purified MT2-A but not MT2-M, to the MT2-A-depleted extract fully reconstitutes methyl-CoM formation from both mono- and dimethylamine Methanosarcina barkeri
2.1.1.247 metabolism MT2-A plays a specific role in metabolism of methylated amine substrates, whereas, MT2-M functions in methane formation from trimethylamine and methanol, while neither of the two MT2 isozymes is involved in methane formation from acetate Methanosarcina barkeri