BRENDA - Enzyme Database

Multiplicity of 3-ketosteroid-9alpha-hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids

Petrusma, M.; Hessels, G.; Dijkhuizen, L.; van der Geize, R.; J. Bacteriol. 193, 3931-3940 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.30
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA1, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshA5, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. kshA5 supports growth on any of the different classes of steroids tested, consistent with its broad expression induction pattern. Recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshB, recombinant expression of kshB in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.30
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol is severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.30
additional information
Rhodococcus rhodochrous
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
1.14.15.30
additional information
Rhodococcus rhodochrous DSM 43269
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.30
Rhodococcus rhodochrous
B6V6V5
isozyme KshA4
-
1.14.15.30
Rhodococcus rhodochrous
F1CMX0
gene kshA1 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA1
-
1.14.15.30
Rhodococcus rhodochrous
F1CMX3
gene kshB, encoding the ferredoxin reductase
-
1.14.15.30
Rhodococcus rhodochrous
F1CMX6
isozyme KshA2
-
1.14.15.30
Rhodococcus rhodochrous
F1CMX8
isozyme KshA3
-
1.14.15.30
Rhodococcus rhodochrous
F1CMY8
gene kshA5 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA5
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
B6V6V5
isozyme KshA4
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
F1CMX0
gene kshA1 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA1
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
F1CMX3
gene kshB, encoding the ferredoxin reductase
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
F1CMX6
isozyme KshA2
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
F1CMX8
isozyme KshA3
-
1.14.15.30
Rhodococcus rhodochrous DSM 43269
F1CMY8
gene kshA5 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA5
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.30
recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshB from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.30
additional information
cell growth on different steroids as a sole carbon and energy source with different isozymes, overview; cell growth on different steroids as a sole carbon and energy source with different isozymes, overview; cell growth on different steroids as a sole carbon and energy source with different isozymes, overview
Rhodococcus rhodochrous
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 43% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 127% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 94% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 101% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 113% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA1 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA3 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA4 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA5 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 13% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 26% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 112% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 99% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 67% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-5alpha-androstane-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 9% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 64% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 19% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 58% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 288% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 194% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 89% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 49% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.30
7
-
assay at; assay at; assay at
Rhodococcus rhodochrous
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.30
[2Fe-2S]-center
-
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.30
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells
Rhodococcus rhodochrous
1.14.15.30
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA1, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
1.14.15.30
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA5, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. kshA5 supports growth on any of the different classes of steroids tested, consistent with its broad expression induction pattern. Recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
1.14.15.30
gene kshB, recombinant expression of kshB in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.30
[2Fe-2S]-center
-
Rhodococcus rhodochrous
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.30
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol is severely impaired, but not completely blocked
Rhodococcus rhodochrous
1.14.15.30
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.30
additional information
Rhodococcus rhodochrous
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
1.14.15.30
additional information
Rhodococcus rhodochrous DSM 43269
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.30
-
Rhodococcus rhodochrous
1.14.15.30
; recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
1.14.15.30
recombinant KshB from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.30
additional information
cell growth on different steroids as a sole carbon and energy source with different isozymes, overview
Rhodococcus rhodochrous
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 43% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 127% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 94% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 101% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 113% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA1 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA3 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA4 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA5 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 13% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 26% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 112% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 99% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 67% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-5alpha-androstane-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 9% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 64% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 19% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 58% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 288% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 194% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 89% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 49% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.30
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous
?
-
-
-
-
1.14.15.30
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
1.14.15.30
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.30
7
-
assay at
Rhodococcus rhodochrous
Expression
EC Number
Organism
Commentary
Expression
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione; in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol; in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol
up
General Information
EC Number
General Information
Commentary
Organism
1.14.15.30
metabolism
3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
1.14.15.30
additional information
KshA and KshB together constitute the KSH enzyme; KshA and KshB together constitute the KSH enzyme; KshA and KshB together constitute the KSH enzyme
Rhodococcus rhodochrous
1.14.15.30
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA1 is dedicated to cholic acid catabolism. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation; 3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA5 appears to be the most versatile enzyme, with the broadest substrate range but without a clear substrate preference. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM 43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.15.30
metabolism
3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
1.14.15.30
additional information
KshA and KshB together constitute the KSH enzyme
Rhodococcus rhodochrous
1.14.15.30
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA1 is dedicated to cholic acid catabolism. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
1.14.15.30
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA5 appears to be the most versatile enzyme, with the broadest substrate range but without a clear substrate preference. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM 43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
1.14.15.30
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid
up
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol
up
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid
up
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione
up
1.14.15.30
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol
up