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Literature summary extracted from
- Differential chitinase activity and production within Francisella species, subspecies, and subpopulations (2011), J. Bacteriol., 193, 3265-3275.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis |
| 3.2.1.14 | expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis subsp. novicida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | additional information | construction of chiA and chiC knockouts strains A1 and A2 strains, respectively | Francisella tularensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
additional information | comparative endochitinase kinetics from different strains, overview | Francisella tularensis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | extracellular | - |
Francisella tularensis subsp. novicida | - |
- |
| 3.2.1.14 | intracellular | - |
Francisella tularensis | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 79000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
| 3.2.1.14 | 97000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
| 3.2.1.14 | 120000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Francisella tularensis | - |
several strains, genes chiA, chiB, chiC, and chiD | - |
| 3.2.1.14 | Francisella tularensis subsp. novicida | - |
different strins, genes chiA, chiB, chiC, and chiD | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis |
| 3.2.1.14 | recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis subsp. novicida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis |
| 3.2.1.14 | additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis subsp. novicida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis | ? | - |
? |  |
| 3.2.1.14 | 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis subsp. novicida | ? | - |
? | |
| 3.2.1.14 | 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 3.2.1.14 | 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 3.2.1.14 | 4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis | 4-nitrophenol + tri-N-acetylchitotriose | - |
? | |
| 3.2.1.14 | 4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + tri-N-acetylchitotriose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ChiA | - |
Francisella tularensis |
| 3.2.1.14 | ChiA | - |
Francisella tularensis subsp. novicida |
| 3.2.1.14 | ChiB | - |
Francisella tularensis |
| 3.2.1.14 | ChiB | - |
Francisella tularensis subsp. novicida |
| 3.2.1.14 | ChiC | - |
Francisella tularensis |
| 3.2.1.14 | ChiC | - |
Francisella tularensis subsp. novicida |
| 3.2.1.14 | ChiD | - |
Francisella tularensis |
| 3.2.1.14 | ChiD | - |
Francisella tularensis subsp. novicida |
| 3.2.1.14 | endochitinase | - |
Francisella tularensis |
| 3.2.1.14 | endochitinase | - |
Francisella tularensis subsp. novicida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 35 | - |
assay at | Francisella tularensis |
| 3.2.1.14 | 35 | - |
assay at | Francisella tularensis subsp. novicida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 7.4 | - |
assay at | Francisella tularensis |
| 3.2.1.14 | 7.4 | - |
assay at | Francisella tularensis subsp. novicida |
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