Any feedback?
Literature summary extracted from
- Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis (2011), FEBS J., 278, 3177-3186.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.1 | expressed in Escherichia coli BL21(DE3) cells | Shewanella amazonensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | D37A | the mutant is completely inactive towards the fluorogenic substrate Mca-KKPAEFFALK-Dnp at pH 4.0 | Shewanella amazonensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.1 | amastatin | 97.2 residual activity at 0.01 mM | Shewanella amazonensis |  |
| 3.4.23.1 | bestatin | 92.7 residual activity at 0.01 mM | Shewanella amazonensis | |
| 3.4.23.1 | dithiothreitol | 91.2 residual activity at 2 mM | Shewanella amazonensis | |
| 3.4.23.1 | E-64 | 89.5 residual activity at 0.01 mM | Shewanella amazonensis | |
| 3.4.23.1 | EDTA | 82.8 residual activity at 5 mM | Shewanella amazonensis | |
| 3.4.23.1 | iodoacetamide | 82.1 residual activity at 0.05 mM | Shewanella amazonensis | |
| 3.4.23.1 | leupeptin | 91.9 residual activity at 0.01 mM | Shewanella amazonensis | |
| 3.4.23.1 | pefabloc | 87.8 residual activity at 1 mM | Shewanella amazonensis | |
| 3.4.23.1 | pepstatin A | complete inhibition at 0.001 mM | Shewanella amazonensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 0.0054 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 50000 | - |
x * 50000, SDS-PAGE | Shewanella amazonensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | Shewanella amazonensis | A1S3N8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.1 | HisTrap column chromatography and Superdex 200 gel filtration | Shewanella amazonensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.1 | Mca-KKPAEFFALK-Dnp + H2O | this peptide is preferentially cleaved at Phe-Phe, with a second minor cleavage occurring at Phe-Ala | Shewanella amazonensis | Mca-KKPAEF + FFALK-Dnp | - |
? | |
| 3.4.23.1 | Mca-KLHPEVLFVLEK-Dnp + H2O | the preferred cleavage site is between Leu-Phe, a minor cleavage site is between Phe-Ala | Shewanella amazonensis | Mca-KLHPEVL + FVLEK-Dnp | - |
? | |
| 3.4.23.1 | additional information | Edans-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-Dabcyl-Arg, Arg-Glu-Edans-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys-Dabcyl-Arg, and Mca-Lys-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp are not significantly processed by shewasin A | Shewanella amazonensis | ? | - |
? | |
| 3.4.23.1 | oxidized insulin B chain + H2O | two major cleavage sites are at Leu15-Tyr16 and Tyr16-Leu17 | Shewanella amazonensis | FVNQHLCGSHLVEAL + L-Tyr + LVCGERGFFYTPKA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | ? | x * 50000, SDS-PAGE | Shewanella amazonensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | shewasin A | - |
Shewanella amazonensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 42 | 50 | maximum activity is observed at temperatures between 42 and 50°C, decreasing so drastically above 50°C that complete loss of activity is detected at 60°C | Shewanella amazonensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 30 | 50 | - |
Shewanella amazonensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 0.03 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 3.8 | 4.5 | - |
Shewanella amazonensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 3.5 | 5 | 50% activity is retained at pH 5.0 At pH 6.0, the enzyme shows no activity | Shewanella amazonensis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 5.6 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
