Literature summary extracted from

Bolscher, J.; Oudhoff, M.; Nazmi, K.; Antos, J.; Guimaraes, C.; Spooner, E.; Haney, E.; Garcia Vallejo, J.; Vogel, H.; Vant Hof, W.; Ploegh, H.; Veerman, E.
Sortase A as a tool for high-yield histatin cyclization (2011), FASEB J., 25, 2650-2658.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.70	expressed in Escherichia coli BL21(DE3) cells	Staphylococcus aureus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.70	Staphylococcus aureus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.70	Ni2+-HisTrap column chromatography	Staphylococcus aureus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.70	Dabcyl-LPETG-EDANS + H2O	-	Staphylococcus aureus	Dabcyl-LPET + Gly-EDANS	-	?
3.4.22.70	additional information	sortase A optimal cleavage site is LPETGG	Staphylococcus aureus	?	-	?
3.4.22.70	additional information	sortase-mediated cyclization of histatin-1 (GG-histatin 1-LPETGG) provides a yield of more than 90%	Staphylococcus aureus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.22.70	SrtA	-	Staphylococcus aureus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)