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Literature summary extracted from

  • Matsushita-Morita, M.; Tada, S.; Suzuki, S.; Hattori, R.; Marui, J.; Furukawa, I.; Yamagata, Y.; Amano, H.; Ishida, H.; Takeuchi, M.; Kashiwagi, Y.; Kusumoto, K.
    Overexpression and characterization of an extracellular leucine aminopeptidase from Aspergillus oryzae (2011), Curr. Microbiol., 62, 557-564.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 overexpressed in Aspergillus oryzae RIB40 Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 bacitracin 87.7% activity at 0.02 mM Aspergillus oryzae
3.4.11.1 bestatin 28.3% activity at 0.02 mM Aspergillus oryzae
3.4.11.1 DTT 3.6% activity at 1 mM Aspergillus oryzae
3.4.11.1 EDTA almost completely inhibited at 1 mM Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.244
-
 L-leucine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae
3.4.11.1 0.782
-
 L-phenylalanine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ 122% activity increase at 1 mM Aspergillus oryzae
3.4.11.1 Zn2+ 258% activity increase at 1 mM Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 33000
-
 x * 33000, SDS-PAGE, mature enzyme has the first 79 N-terminal amino acid residues removed (N-terminal sequencing) Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Aspergillus oryzae
-
-
-
3.4.11.1 Aspergillus oryzae RIB 40
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.1 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 from culture supernatant, ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 0.8
-
 Leu-Leu-Leu, pH 8.5, 50°C Aspergillus oryzae
3.4.11.1 142
-
 L-leucine-4-nitroanilide, pH 8.5, 50°C, 50% activity in the presence of 1 M NaCl Aspergillus oryzae
3.4.11.1 202
-
 L-leucine-4-nitroanilide, pH 8.5, 60°C Aspergillus oryzae
3.4.11.1 221
-
 Leu-Arg-Pro-Gly, pH 8.5, 50°C Aspergillus oryzae
3.4.11.1 262.3
-
 Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu, pH 8.5, 50°C Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-arginine-4-nitroanilide + H2O 16.5% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae L-arginine + 4-nitroaniline
-
 ?
3.4.11.1 L-leucine-4-nitroanilide + H2O
-
 Aspergillus oryzae L-leucine + 4-nitroaniline
-
 ?
3.4.11.1 L-leucine-4-nitroanilide + H2O
-
 Aspergillus oryzae RIB 40 L-leucine + 4-nitroaniline
-
 ?
3.4.11.1 L-lysine-4-nitroanilide + H2O 24.5% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae L-lysine + 4-nitroaniline
-
 ?
3.4.11.1 L-lysine-4-nitroanilide + H2O 24.5% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae RIB 40 L-lysine + 4-nitroaniline
-
 ?
3.4.11.1 L-methionine-4-nitroanilide + H2O 29.6% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae L-methionine + 4-nitroaniline
-
 ?
3.4.11.1 L-methionine-4-nitroanilide + H2O 29.6% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae RIB 40 L-methionine + 4-nitroaniline
-
 ?
3.4.11.1 L-phenylalanine-4-nitroanilide + H2O 38.8% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae L-phenylalanine + 4-nitroaniline
-
 ?
3.4.11.1 L-phenylalanine-4-nitroanilide + H2O 38.8% activity in comparison to L-leucine-4-nitroanilide Aspergillus oryzae RIB 40 L-phenylalanine + 4-nitroaniline
-
 ?
3.4.11.1 Leu-Arg-Pro-Gly + H2O
-
 Aspergillus oryzae Leu + Arg-Pro-Gly
-
 ?
3.4.11.1 Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu + H2O
-
 Aspergillus oryzae Leu + Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu
-
 ?
3.4.11.1 Leu-Leu-Leu + H2O
-
 Aspergillus oryzae Leu + Leu-Leu
-
 ?
3.4.11.1 Leu-Leu-Leu + H2O
-
 Aspergillus oryzae RIB 40 Leu + Leu-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 33000, SDS-PAGE, mature enzyme has the first 79 N-terminal amino acid residues removed (N-terminal sequencing) Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 leucine aminopeptidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 60
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 60
-
 stable up to 60°C, inactive at 70°C Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 37
-
 L-phenylalanine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae
3.4.11.1 50.9
-
 L-leucine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.5
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 7.5 11 more than 80% activity and stable Aspergillus oryzae

Expression

EC Number Organism Comment Expression
3.4.11.1 Aspergillus oryzae under alkaline condition (pH 10.0) up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 51.68
-
 L-phenylalanine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae
3.4.11.1 209
-
 L-leucine-4-nitroanilide pH 8.5, 50°C Aspergillus oryzae