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Literature summary extracted from

  • Kondo, K.; Hiyama, Y.; Matsumoto, M.
    Kinetics of hydrolysis reaction of chitin oligomer by a chitinase purified from Bacillus subtilis (2011), Chem. Eng. Transact., 24, 877-882.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information Michaelis-Menten kinetics, overview Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 30000
-
 x * 85000, Chi1, SDS-PAGE, x * 30000, Chi2, SDS-PAGE, x * 55000, Chi3, SDS-PAGE Bacillus subtilis
3.2.1.14 55000
-
 x * 85000, Chi1, SDS-PAGE, x * 30000, Chi2, SDS-PAGE, x * 55000, Chi3, SDS-PAGE Bacillus subtilis
3.2.1.14 85000
-
 x * 85000, Chi1, SDS-PAGE, x * 30000, Chi2, SDS-PAGE, x * 55000, Chi3, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus subtilis
-
 isolated from natto, a traditional Japanese food, 3 isozymes Chi1-3
-
3.2.1.14 Bacillus subtilis 1076
-
 isolated from natto, a traditional Japanese food, 3 isozymes Chi1-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native extracellular enzyme from culture medium by anion exchange chromatography Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 additional information carbon sources are colloidal chitin and chitin Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.14 chitin + H2O
-
 Bacillus subtilis 1076 ?
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O Chi3 Bacillus subtilis N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O Chi3 Bacillus subtilis 1076 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O Chi3 Bacillus subtilis 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O Chi3 Bacillus subtilis 1076 2 N,N'-diacetylchitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 85000, Chi1, SDS-PAGE, x * 30000, Chi2, SDS-PAGE, x * 55000, Chi3, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25
-
 Chi1 Bacillus subtilis
3.2.1.14 37
-
 Chi2 and Chi3 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4
-
 Chi2 and Chi3 Bacillus subtilis
3.2.1.14 8
-
 Chi1 Bacillus subtilis