Literature summary extracted from
Kim, Y.S.; Park, C.S.; Oh, D.K.
Retinal production from beta-carotene by beta-carotene 15,15-dioxygenase from an unculturable marine bacterium (2010), Biotechnol. Lett., 32, 957-961.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.13.11.63 |
synthesis |
production of all-trans-retinal by recombinant enzyme. Toluene is an optimal solvent for the dissolution of beta-carotene, and the optimal solution for the conversion reaction contains 2.4% (w/v) Tween 20, 0.15 U enzyme/ml, and 350 mg beta-carotene/l. Under these conditions, the enzyme produces 181 mg retinal/l after 20 h |
uncultured marine bacterium |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.63 |
expression in Escherichia coli |
uncultured marine bacterium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.63 |
uncultured marine bacterium |
Q4PNI0 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.63 |
beta-carotene + O2 |
- |
uncultured marine bacterium |
2 all-trans-retinal |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.63 |
BLH |
- |
uncultured marine bacterium |