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Literature summary extracted from

  • Okamoto, D.N.; Oliveira, L.C.; Kondo, M.Y.; Cezari, M.H.; Szeltner, Z.; Juhasz, T.; Juliano, M.A.; Polgar, L.; Juliano, L.; Gouvea, I.E.
    Increase of SARS-CoV 3CL peptidase activity due to macromolecular crowding effects in the milieu composition (2010), Biol. Chem., 391, 1461-1468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.69 bovine serum albumin 10% (w/v) bovine serum albumin improves the hydrolytic rate by a factor of 2 Severe acute respiratory syndrome-related coronavirus
3.4.22.69 PEG6000 3fold activation in the presence of 10% (w/v) PEG6000 Severe acute respiratory syndrome-related coronavirus
3.4.22.69 Sodium sulfate 1 M sodium sulfate stimulates activity Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.69 expressed in Escherichia coli Rosetta (DE3) cells Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.69 0.0005
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0011
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0013
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0013
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0016
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0017
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.002
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0024
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.011
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.0136
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.016
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.023
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.033
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.69 additional information the enzyme activity is unaffected by Brij-35, Triton X-100 and Tween 20 or SDS Severe acute respiratory syndrome-related coronavirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.69 Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.69 Resource-Q column chromatography, gel filtration Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.69 Abz-LQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-LQ + SGFRK(Dnp)NH2
-
 ?
3.4.22.69 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-LYQPPQTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
3.4.22.69 Abz-QTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-QTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
3.4.22.69 Abz-SAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRK(Dnp)NH2
-
 ?
3.4.22.69 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAFPSGK(Dnp)NH2
-
 ?
3.4.22.69 Abz-SAVLQSGFRKMAK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAK(Dnp)NH2
-
 ?
3.4.22.69 Abz-SAVLQSGK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGK(Dnp)NH2
-
 ?
3.4.22.69 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SGADVLYQPPQTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
3.4.22.69 Abz-VLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-VLQ + SGFRK(Dnp)NH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.69 3cLpro
-
 Severe acute respiratory syndrome-related coronavirus
3.4.22.69 SARS-CoV 3CL peptidase
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.69 0.003
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.004
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.02
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.04
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.07
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.11
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.28
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.38
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.6
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.77
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 2.9
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 3
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 3.3
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.69 7.5
-
-
 Severe acute respiratory syndrome-related coronavirus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.69 6.5 8.5
-
 Severe acute respiratory syndrome-related coronavirus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.69 0.13
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.31
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.2
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 4.4
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 12
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 69
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 345
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 545
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 560
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 592
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1440
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1500
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.4.22.69 2250
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus